Literature DB >> 19800315

Role of galE on biofilm formation by Thermus spp.

Yi-Kai Niou1, Wan-Ling Wu, Ling-Chun Lin, Mei-Shiuan Yu, Hung-Yu Shu, Hsueh-Hui Yang, Guang-Huey Lin.   

Abstract

Thermus thermophilus and Thermus aquaticus are thermophilic bacteria that are frequently found to attach to solid surfaces in hot springs to form biofilms. Uridine diphosphate (UDP)-galactose-4'-epimerase (GalE) is an enzyme that catalyzes the conversion of UDP-galactose to UDP-glucose, an important biochemical step in exopolysaccharide synthesis. We expressed GalE obtained from T. thermophilus HB8 in Escherichia coli and found that the enzyme is stable at 80 degrees C and can epimerize UDP-galactose to UDP-glucose and UDP-N-acetylgalactosamine (UDP-GalNAc) to UDP-N-acetylglucosamine (UDP-GlcNAc). Enzyme overexpression in T. thermophilus HB27 led to an increased capacity of biofilm production. Therefore, the galE gene is important to biofilm formation because of its involvement in epimerizing UDP-galactose and UDP-N-acetylgalactosamine for exopolysaccharide biosynthesis.

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Year:  2009        PMID： 19800315     DOI： 10.1016/j.bbrc.2009.09.120

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  8 in total

1.  The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination.

Authors:  Naoto Ohtani; Masaru Tomita; Mitsuhiro Itaya
Journal:  Extremophiles       Date:  2012-01-03       Impact factor: 2.395

2.  Phosphoproteomic analysis reveals the effects of PilF phosphorylation on type IV pilus and biofilm formation in Thermus thermophilus HB27.

Authors:  Wan-Ling Wu; Jiahn-Haur Liao; Guang-Huey Lin; Miao-Hsia Lin; Ying-Che Chang; Suh-Yuen Liang; Feng-Ling Yang; Kay-Hooi Khoo; Shih-Hsiung Wu
Journal:  Mol Cell Proteomics       Date:  2013-07-04       Impact factor: 5.911

3.  iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae.

Authors:  Yan Wang; Shan Cong; Qinghua Zhang; Ranwei Li; Ke Wang
Journal:  Infect Drug Resist       Date:  2020-08-19       Impact factor: 4.003

4.  Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis.

Authors:  Miriam Edel; Gunnar Sturm; Katrin Sturm-Richter; Michael Wagner; Julia Novion Ducassou; Yohann Couté; Harald Horn; Johannes Gescher
Journal:  Biotechnol Biofuels       Date:  2021-06-03       Impact factor: 6.040

5.  Metagenomic analysis of ammonia-oxidizing archaea affiliated with the soil group.

Authors:  Rita Bartossek; Anja Spang; Gerhard Weidler; Anders Lanzen; Christa Schleper
Journal:  Front Microbiol       Date:  2012-06-20       Impact factor: 5.640

6.  Pseudomonas aeruginosa isolates from dental unit waterlines can be divided in two distinct groups, including one displaying phenotypes similar to isolates from cystic fibrosis patients.

Authors:  Myriam M Ouellet; Annie Leduc; Christine Nadeau; Jean Barbeau; Steve J Charette
Journal:  Front Microbiol       Date:  2015-01-21       Impact factor: 5.640

7.  Quorum sensing in thermophiles: prevalence of autoinducer-2 system.

Authors:  Amandeep Kaur; Neena Capalash; Prince Sharma
Journal:  BMC Microbiol       Date:  2018-06-28       Impact factor: 3.605

8.  Toward repurposing ciclopirox as an antibiotic against drug-resistant Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae.

Authors:  Kimberly M Carlson-Banning; Andrew Chou; Zhen Liu; Richard J Hamill; Yongcheng Song; Lynn Zechiedrich
Journal:  PLoS One       Date:  2013-07-23       Impact factor: 3.240
  8 in total

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