| Literature DB >> 19799181 |
Cyril Buhler1, Robert Shroff, Michael Lichten.
Abstract
DNA double-strand breaks (DSBs) initiate meiotic recombination in eukaryotes. We describe two strategies that use microarrays to determine the genome-wide distribution of meiotic DSBs in the yeast Saccharomyces cerevisiae. The first is a chromatin immunoprecipitation (ChIP) approach that targets the Spo11 protein, which remains covalently attached to DSB ends in certain mutant backgrounds. The second approach involves BND cellulose enrichment of the single-strand DNA (ssDNA) recombination intermediate formed by end-resection at DSB sites following Spo11 removal.Entities:
Mesh：
Year: 2009 PMID： 19799181 DOI： 10.1007/978-1-59745-527-5_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745