Spectral resolution of molecular ensembles under ambient conditions using surface plasmon coupled fluorescence emission.

We report the ability to spectrally resolve excited-state ensembles of pyranine (Py) utilizing nanometer-thick metal films as a low-cost analytical tool. Surface plasmon coupling allows to mitigate the effect of spectral broadening that is responsible for blurring the emission spectrum at room temperature, a situation common in conventional fluorescence spectroscopy. The approach is especially useful in the case when several excited-state species are present. Fluorescence emission from closely located protonated, deprotonated, and excimer species of Py couple into surface plasmons and are easily separated and observed with 11-14 fold intensity enhancements. Furthermore, the ultranarrowband photon-sorting of emission from microenvironments in a multispecies system is performed in this study using instruments that are readily available in most laboratories without employing any deconvolution procedure and/or additional dispersive optics.