R Vimalavathini1, B Gitanjali. 1. Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education & Research Puducherry, India.
Abstract
BACKGROUND & OBJECTIVE: Since proper storage of insulin is necessary for its action, the present study was undertaken to determine the extent to which improper temperature storage conditions could have contributed to the potency of the three insulin formulations tested. METHODS: Two human insulin formulations (regular and biphasic) from three different manufacturers were stored at 5 different temperatures. In vitro potency of insulin was determined by high performance liquid chromatography on every seventh day for a period of 28 days. For the in vivo study, insulin tolerance test was done by injecting human regular insulin intraperitoneally to rabbits on the 25(th) day of storage. Blood glucose was determined at 0, 15, 30 and 60 min after insulin injection using glucometer. RESULTS: Storage at 32 and 37 degrees C showed 14-18 per cent decrease in potency of insulin in both the formulations on 28(th) day for all the three brands. Also the rabbits receiving insulin stored in 32 and 37 degrees C did not show a significant decrease in blood sugar level when compared to those receiving insulin stored at 5 degrees C. INTERPRETATION & CONCLUSION: Improper storage of insulin decreases the potency and hence the pharmacological action of insulin. Patients should be educated on the proper methods of storage, and free supplies of insulin for more than two weeks use should not be dispensed.
BACKGROUND & OBJECTIVE: Since proper storage of insulin is necessary for its action, the present study was undertaken to determine the extent to which improper temperature storage conditions could have contributed to the potency of the three insulin formulations tested. METHODS: Two humaninsulin formulations (regular and biphasic) from three different manufacturers were stored at 5 different temperatures. In vitro potency of insulin was determined by high performance liquid chromatography on every seventh day for a period of 28 days. For the in vivo study, insulin tolerance test was done by injecting human regular insulin intraperitoneally to rabbits on the 25(th) day of storage. Blood glucose was determined at 0, 15, 30 and 60 min after insulin injection using glucometer. RESULTS: Storage at 32 and 37 degrees C showed 14-18 per cent decrease in potency of insulin in both the formulations on 28(th) day for all the three brands. Also the rabbits receiving insulin stored in 32 and 37 degrees C did not show a significant decrease in blood sugar level when compared to those receiving insulin stored at 5 degrees C. INTERPRETATION & CONCLUSION: Improper storage of insulin decreases the potency and hence the pharmacological action of insulin. Patients should be educated on the proper methods of storage, and free supplies of insulin for more than two weeks use should not be dispensed.
Authors: Abm Kamrul-Hasan; Ajit Kumar Paul; Mohammad Nurul Amin; Md Abu Jar Gaffar; Md Asaduzzaman; Mohammad Saifuddin; Marufa Mustari; Md Jahangir Alam; Mohammad Moin Shahid; K M Nahid-Ul-Haque; Muhammad Shah Alam; Md Motiur Rahman; Samir Kumar Talukder; Md Abdul Kader; Farhana Akter; Mohammad Abdul Hannan; Palash Kumar Chanda; Muhammed Abu Bakar; Shahjada Selim Journal: Eur Endocrinol Date: 2020-02-28
Authors: Jeremy Joslin; Martin D Hoffman; Ian Rogers; Robert M Worthing; Matt Ladbrook; Joshua Mularella Journal: Sports Med Date: 2015-08 Impact factor: 11.136
Authors: Steven Ross; Nicolaos Scoutaris; Dimitrios Lamprou; David Mallinson; Dennis Douroumis Journal: Drug Deliv Transl Res Date: 2015-08 Impact factor: 4.617