AIMS: It has long been demonstrated that epidermal growth factor (EGF) has catabolic effects on bone. Thus, we examined the role of EGF in regulating mechanically induced bone modeling in a rat model of orthodontic tooth movement. MAIN METHODS: The maxillary first molars of rats were moved mesially using an orthodontic appliance attached to the maxillary incisor teeth. Rats were randomly divided into 4 groups: (G1) administration of PBS (phosphate buffer saline) solution (n=24); (G2) administration of empty liposomes (n=24); (G3) administration 20ng of EGF solution (n=24); and (G4) 20ng of EGF-liposomes solution (n=24). Each solution was injected in the mucosa of the left first molar adjacent to the appliance. At days 5, 10, 14 and 21 after drug administration, 6 animals of each group were sacrificed. Histomorphometric analysis was used to quantify osteoclasts (Tartrate-resistant acid phosphatase (TRAP)+cells) and tooth movement. Using immunohistochemistry assay we evaluated the RANKL (receptor activator of nuclear factor kappaB ligand) and epidermal growth factor receptor (EGFR) expression. KEY FINDINGS: The EGF-liposome administration showed an increased tooth movement and osteoclast numbers compared to controls (p<0.05). This was correlated with intense RANKL expression. Both osteoblasts and osteoclasts expressed EGFR. SIGNIFICANCE: Local delivery of EGF-liposome stimulates osteoclastogenesis and tooth movement.
AIMS: It has long been demonstrated that epidermal growth factor (EGF) has catabolic effects on bone. Thus, we examined the role of EGF in regulating mechanically induced bone modeling in a rat model of orthodontic tooth movement. MAIN METHODS: The maxillary first molars of rats were moved mesially using an orthodontic appliance attached to the maxillary incisor teeth. Rats were randomly divided into 4 groups: (G1) administration of PBS (phosphate buffer saline) solution (n=24); (G2) administration of empty liposomes (n=24); (G3) administration 20ng of EGF solution (n=24); and (G4) 20ng of EGF-liposomes solution (n=24). Each solution was injected in the mucosa of the left first molar adjacent to the appliance. At days 5, 10, 14 and 21 after drug administration, 6 animals of each group were sacrificed. Histomorphometric analysis was used to quantify osteoclasts (Tartrate-resistant acid phosphatase (TRAP)+cells) and tooth movement. Using immunohistochemistry assay we evaluated the RANKL (receptor activator of nuclear factor kappaB ligand) and epidermal growth factor receptor (EGFR) expression. KEY FINDINGS: The EGF-liposome administration showed an increased tooth movement and osteoclast numbers compared to controls (p<0.05). This was correlated with intense RANKL expression. Both osteoblasts and osteoclasts expressed EGFR. SIGNIFICANCE: Local delivery of EGF-liposome stimulates osteoclastogenesis and tooth movement.
Authors: Nunzio Cirulli; Alessio Danilo Inchingolo; Assunta Patano; Sabino Ceci; Grazia Marinelli; Giuseppina Malcangi; Giovanni Coloccia; Valentina Montenegro; Chiara Di Pede; Anna Maria Ciocia; Giuseppe Barile; Antonio Mancini; Giulia Palmieri; Daniela Azzollini; Biagio Rapone; Ludovica Nucci; Ioana Roxana Bordea; Antonio Scarano; Felice Lorusso; Gianluca Martino Tartaglia; Cinzia Maspero; Manuel Nuzzolese; Filippo Cardarelli; Daniela Di Venere; Angelo Michele Inchingolo; Gianna Dipalma; Francesco Inchingolo Journal: Int J Environ Res Public Health Date: 2022-06-17 Impact factor: 4.614
Authors: Chung How Kau; Alpdogan Kantarci; Tim Shaughnessy; Amornpong Vachiramon; Peerapong Santiwong; Alvaro de la Fuente; Darya Skrenes; Dennis Ma; Peter Brawn Journal: Prog Orthod Date: 2013-09-19 Impact factor: 2.750