PURPOSE: We tested a new light detection cooled charge-coupled device (CCD) for in vivo assessment of noninvasive, whole-body fluorescence optical imaging of adenovirus directed enhanced green fluorescent protein (AdEGFP) expression. PROCEDURES: AdEGFP was injected i.v. into BALB/c mice via tail vein. Whole-body fluorescence optical imaging of AdEGFP expression was performed using a Kodak 2000MM Image Station before and after vector administration. RESULTS: EGFP expression was exclusively detected around the abdominal cavity, and the fluorescent signal peaked at day 4 and then remained detectable for at least 30 days. Ex vivo fluorescence imaging confirmed that EGFP expression was restricted to the liver, and transgene expression was homogeneously diffused into all four lobes. CONCLUSIONS: These findings demonstrate that in vivo fluorescence imaging provides functional data indicating the approximate location, magnitude, and duration of AdEGFP expression.
PURPOSE: We tested a new light detection cooled charge-coupled device (CCD) for in vivo assessment of noninvasive, whole-body fluorescence optical imaging of adenovirus directed enhanced green fluorescent protein (AdEGFP) expression. PROCEDURES: AdEGFP was injected i.v. into BALB/c mice via tail vein. Whole-body fluorescence optical imaging of AdEGFP expression was performed using a Kodak 2000MM Image Station before and after vector administration. RESULTS: EGFP expression was exclusively detected around the abdominal cavity, and the fluorescent signal peaked at day 4 and then remained detectable for at least 30 days. Ex vivo fluorescence imaging confirmed that EGFP expression was restricted to the liver, and transgene expression was homogeneously diffused into all four lobes. CONCLUSIONS: These findings demonstrate that in vivo fluorescence imaging provides functional data indicating the approximate location, magnitude, and duration of AdEGFP expression.
Authors: Jusan Yang; Jennifer J Marden; Chenguang Fan; Salih Sanlioglu; Robert M Weiss; Teresa C Ritchie; Robin L Davisson; John F Engelhardt Journal: Mol Ther Date: 2003-03 Impact factor: 11.454
Authors: Kevin Doerschug; Salih Sanlioglu; Dawn M Flaherty; Rebecca L Wilson; Timur Yarovinsky; Martha M Monick; John F Engelhardt; Gary W Hunninghake Journal: J Immunol Date: 2002-12-01 Impact factor: 5.422
Authors: Daria A Burmistrova; Sergey V Tillib; Dmitry V Shcheblyakov; Inna V Dolzhikova; Dmitry N Shcherbinin; Olga V Zubkova; Tatiana I Ivanova; Amir I Tukhvatulin; Maxim M Shmarov; Denis Y Logunov; Boris S Naroditsky; Aleksandr L Gintsburg Journal: PLoS One Date: 2016-03-10 Impact factor: 3.240