Literature DB >> 19788886

Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.

Mitri K Khoury1, Ian Parker, Dana W Aswad.   

Abstract

We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associated with processing film. Copyright 2009 Elsevier Inc. All rights reserved.

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Year:  2009        PMID： 19788886      PMCID： PMC2808431          DOI： 10.1016/j.ab.2009.09.041

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  30 in total

1.  Inactivation of the budded virus of Autographa californica M nucleopolyhedrovirus by gloverin.

Authors:  Daniela A Moreno-Habel; Ivan M Biglang-awa; Angelica Dulce; Dee Dee Luu; Peter Garcia; Paul M M Weers; Eric J Haas-Stapleton
Journal:  J Invertebr Pathol       Date:  2012-02-28       Impact factor: 2.841

2.  Type II Toxoplasma gondii induction of CD40 on infected macrophages enhances interleukin-12 responses.

Authors:  Pedro Morgado; Dattanand M Sudarshana; Lanny Gov; Katherine S Harker; Tonika Lam; Paolo Casali; Jon P Boyle; Melissa B Lodoen
Journal:  Infect Immun       Date:  2014-07-14       Impact factor: 3.441

3.  Complement protein C1q-mediated neuroprotection is correlated with regulation of neuronal gene and microRNA expression.

Authors:  Marie E Benoit; Andrea J Tenner
Journal:  J Neurosci       Date:  2011-03-02       Impact factor: 6.167

4.  Serological Survey on Bacterial and Viral Pathogens in Wild Boars Hunted in Tuscany.

Authors:  Fabrizio Bertelloni; Maurizio Mazzei; Giovanni Cilia; Mario Forzan; Antonio Felicioli; Simona Sagona; Patrizia Bandecchi; Barbara Turchi; Domenico Cerri; Filippo Fratini
Journal:  Ecohealth       Date:  2020-02-07       Impact factor: 3.184

5.  Phosphoinositide-interacting regulator of TRP (PIRT) has opposing effects on human and mouse TRPM8 ion channels.

Authors:  Jacob K Hilton; Taraneh Salehpour; Nicholas J Sisco; Parthasarathi Rath; Wade D Van Horn
Journal:  J Biol Chem       Date:  2018-05-03       Impact factor: 5.157

6.  mTORC1 Inhibition Induces Resistance to Methotrexate and 6-Mercaptopurine in Ph+ and Ph-like B-ALL.

Authors:  Thanh-Trang T Vo; J Scott Lee; Duc Nguyen; Brandon Lui; William Pandori; Andrew Khaw; Sharmila Mallya; Mengrou Lu; Markus Müschen; Marina Konopleva; David A Fruman
Journal:  Mol Cancer Ther       Date:  2017-05-31       Impact factor: 6.261

7.  Isoaspartyl protein damage and repair in mouse retina.

Authors:  Zhenxia Qin; Jing Yang; Henry J Klassen; Dana W Aswad
Journal:  Invest Ophthalmol Vis Sci       Date:  2014-03-13       Impact factor: 4.799

8.  Atomic-resolution structure of a disease-relevant Aβ(1-42) amyloid fibril.

Authors:  Marielle Aulikki Wälti; Francesco Ravotti; Hiromi Arai; Charles G Glabe; Joseph S Wall; Anja Böckmann; Peter Güntert; Beat H Meier; Roland Riek
Journal:  Proc Natl Acad Sci U S A       Date:  2016-07-28       Impact factor: 11.205

9.  C1q-induced LRP1B and GPR6 proteins expressed early in Alzheimer disease mouse models, are essential for the C1q-mediated protection against amyloid-β neurotoxicity.

Authors:  Marie E Benoit; Michael X Hernandez; Minhan L Dinh; Francisca Benavente; Osvaldo Vasquez; Andrea J Tenner
Journal:  J Biol Chem       Date:  2012-11-13       Impact factor: 5.157

Review 10.  The necessity of and strategies for improving confidence in the accuracy of western blots.

Authors:  Rajeshwary Ghosh; Jennifer E Gilda; Aldrin V Gomes
Journal:  Expert Rev Proteomics       Date:  2014-07-25       Impact factor: 3.940
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