Literature DB >> 1978720

The OLE1 gene of Saccharomyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-CoA desaturase gene.

J E Stukey1, V M McDonough, C E Martin.   

Abstract

Strains of Saccharomyces cerevisiae bearing the ole1 mutation are defective in unsaturated fatty acid (UFA) synthesis and require UFAs for growth. A previously isolated yeast genomic fragment complementing the ole1 mutation has been sequenced and determined to encode the delta 9 fatty acid desaturase enzyme by comparison of primary amino acid sequence to the rat liver stearoyl-CoA desaturase. The OLE1 structural gene encodes a protein of 510 amino acids (251 hydrophobic) having an approximate molecular mass of 57.4 kDa. A 257-amino acid internal region of the yeast open reading frame aligns with and shows 36% identity and 60% similarity to the rat liver stearoyl-CoA desaturase protein. This comparison disclosed three short regions of high consecutive amino acid identity (greater than 70%) including one 11 of 12 perfect residue match. The predicted yeast enzyme contains at least four potential membrane-spanning regions and several shorter hydrophobic regions that align exactly with similar sequences in the rat liver protein. An ole1 gene-disrupted yeast strain was transformed with a yeast-rat chimeric gene consisting of the promoter region and N-terminal 27 codons of OLE1 fused to the rat desaturase coding sequence. Fusion gene transformants displayed near equivalent growth rates and modest lipid composition changes relative to wild type yeast control implying a significant conservation of delta 9 desaturase tertiary structure and efficient interaction between the rat desaturase and yeast cytochrome b5.

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Year:  1990        PMID: 1978720

Source DB:  PubMed          Journal:  J Biol Chem        ISSN: 0021-9258            Impact factor:   5.157


  119 in total

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Journal:  Microbiol Mol Biol Rev       Date:  2000-06       Impact factor: 11.056

2.  Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites.

Authors:  L Zhang; L Ge; S Parimoo; K Stenn; S M Prouty
Journal:  Biochem J       Date:  1999-05-15       Impact factor: 3.857

3.  Molecular cloning of delta 9 fatty acid desaturase from the protozoan Tetrahymena thermophila and its mRNA expression during thermal membrane adaptation.

Authors:  S Nakashima; Y Zhao; Y Nozawa
Journal:  Biochem J       Date:  1996-07-01       Impact factor: 3.857

4.  Identification and characterization of stearoyl-CoA desaturase in Toxoplasma gondii.

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Journal:  Acta Biochim Biophys Sin (Shanghai)       Date:  2019-06-20       Impact factor: 3.848

5.  Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells.

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Journal:  Biochem J       Date:  2005-05-15       Impact factor: 3.857

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7.  Evolution-related amino acids play important role in determining regioselectivity of fatty acid desaturase from Pichia pastoris.

Authors:  Xinxin Zhang; Dongsheng Wei; Mingchun Li; Yuyan Qi; Laijun Xing
Journal:  Mol Biol Rep       Date:  2008-02-20       Impact factor: 2.316

8.  Expression of the Yeast Delta-9 Fatty Acid Desaturase in Nicotiana tabacum.

Authors:  J J Polashock; C K Chin; C E Martin
Journal:  Plant Physiol       Date:  1992-10       Impact factor: 8.340

9.  Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress.

Authors:  Sonia Rodríguez-Vargas; Alicia Sánchez-García; Jose Manuel Martínez-Rivas; Jose Antonio Prieto; Francisca Randez-Gil
Journal:  Appl Environ Microbiol       Date:  2006-10-27       Impact factor: 4.792

10.  Merging of multiple signals regulating delta9 fatty acid desaturase gene transcription in Saccharomyces cerevisiae.

Authors:  Y Nakagawa; A Ueda; Y Kaneko; S Harashima
Journal:  Mol Genet Genomics       Date:  2003-05-06       Impact factor: 3.291

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