OBJECTIVE: Determine the prevalence of bacterial biofilms in surgical chronic rhinosinusitis (CRS) patients and characterize the inflammatory response associated with biofilm CRS. STUDY DESIGN: Cross-sectional. SETTING: Tertiary care academic center. SUBJECTS AND METHODS: Sinonasal mucosa and peripheral blood were collected from 60 CRS patients. Mucosal biofilms were demonstrated by scanning electron microscopy. Leukocyte subpopulations were determined by flow cytometry. Cytokines were identified with a luminex-based assay on the lysate of homogenized tissue or plasma. RESULTS: Of the 60 samples, 17 were determined to be positive for the presence of biofilms. Oral steroid-naive CRS patients with biofilm demonstrated a local T(H)1 inflammatory response with significantly elevated levels of interferon-gamma (INF-gamma), granulocyte colony-stimulating factor, macrophage inflammatory protein-1 beta, and neutrophils in the sinonasal mucosa. No differences were present at the systemic level. CONCLUSION: Sinonasal bacterial biofilms correlate to a T(H)1 skewed local but not systemic inflammatory response in CRS. This difference is abrogated by the use of oral steroids.
OBJECTIVE: Determine the prevalence of bacterial biofilms in surgical chronic rhinosinusitis (CRS) patients and characterize the inflammatory response associated with biofilm CRS. STUDY DESIGN: Cross-sectional. SETTING: Tertiary care academic center. SUBJECTS AND METHODS: Sinonasal mucosa and peripheral blood were collected from 60 CRSpatients. Mucosal biofilms were demonstrated by scanning electron microscopy. Leukocyte subpopulations were determined by flow cytometry. Cytokines were identified with a luminex-based assay on the lysate of homogenized tissue or plasma. RESULTS: Of the 60 samples, 17 were determined to be positive for the presence of biofilms. Oral steroid-naive CRSpatients with biofilm demonstrated a local T(H)1 inflammatory response with significantly elevated levels of interferon-gamma (INF-gamma), granulocyte colony-stimulating factor, macrophage inflammatory protein-1 beta, and neutrophils in the sinonasal mucosa. No differences were present at the systemic level. CONCLUSION: Sinonasal bacterial biofilms correlate to a T(H)1 skewed local but not systemic inflammatory response in CRS. This difference is abrogated by the use of oral steroids.
Authors: M Skolimowski; M Weiss Nielsen; F Abeille; P Skafte-Pedersen; D Sabourin; A Fercher; D Papkovsky; S Molin; R Taboryski; C Sternberg; M Dufva; O Geschke; J Emnéus Journal: Biomicrofluidics Date: 2012-08-02 Impact factor: 2.800
Authors: Robert J Lee; Guoxiang Xiong; Jennifer M Kofonow; Bei Chen; Anna Lysenko; Peihua Jiang; Valsamma Abraham; Laurel Doghramji; Nithin D Adappa; James N Palmer; David W Kennedy; Gary K Beauchamp; Paschalis-Thomas Doulias; Harry Ischiropoulos; James L Kreindler; Danielle R Reed; Noam A Cohen Journal: J Clin Invest Date: 2012-10-08 Impact factor: 14.808