BACKGROUND: Bronchoalveolar lavage fluid (BALF) is an important diagnostic source to investigate molecular changes occurring in lung disorders. The objective of this study was to assess and compare the peptidomic profiles of BALF from premature neonates with and without bronchopulmonary dysplasia (BPD). METHODS: Samples were obtained on the 3rd day of life from 34 neonates with gestational age <or=32 weeks. Two pools of samples from patients with and without BPD were analyzed by high performance liquid chromatography. Several differentially expressed peptides were collected and sequenced. Moreover, samples from single donors were analyzed by liquid chromatography-electrospray ionization mass spectrometry to define the molecular mass values of various peptides and to quantify their expression. Levels of some matrix metalloproteinases and their tissue inhibitors were also determined in single samples. RESULTS: Neonates of the BPD group (N = 16) showed significantly lower mean gestational age and birth weight with respect to the no-BPD group (N = 18; P < 0.0001). Levels of six peptides were significantly higher in BPD patients (P < 0.05). Two of them were identified as the albumin fragments 1-21 (2,428 Da) and 399-406 (956 Da). Levels of matrix metalloproteinase-3 (MMP-3) enzyme probably involved in albumin fragment generation were also significantly higher in the BPD group compared to the no-BPD group (P < 0.05), whereas the levels of tissue inhibitor of metalloproteinases-1 were significantly lower (P < 0.05). Levels of albumin fragments and MMP-3 showed a significant correlation (P < 0.05). CONCLUSIONS: This study shows that proteomic techniques can be applied to investigate the involvement of proteolytic enzymes on the airways of mechanically ventilated premature infants.
BACKGROUND: Bronchoalveolar lavage fluid (BALF) is an important diagnostic source to investigate molecular changes occurring in lung disorders. The objective of this study was to assess and compare the peptidomic profiles of BALF from premature neonates with and without bronchopulmonary dysplasia (BPD). METHODS: Samples were obtained on the 3rd day of life from 34 neonates with gestational age <or=32 weeks. Two pools of samples from patients with and without BPD were analyzed by high performance liquid chromatography. Several differentially expressed peptides were collected and sequenced. Moreover, samples from single donors were analyzed by liquid chromatography-electrospray ionization mass spectrometry to define the molecular mass values of various peptides and to quantify their expression. Levels of some matrix metalloproteinases and their tissue inhibitors were also determined in single samples. RESULTS: Neonates of the BPD group (N = 16) showed significantly lower mean gestational age and birth weight with respect to the no-BPD group (N = 18; P < 0.0001). Levels of six peptides were significantly higher in BPD patients (P < 0.05). Two of them were identified as the albumin fragments 1-21 (2,428 Da) and 399-406 (956 Da). Levels of matrix metalloproteinase-3 (MMP-3) enzyme probably involved in albumin fragment generation were also significantly higher in the BPD group compared to the no-BPD group (P < 0.05), whereas the levels of tissue inhibitor of metalloproteinases-1 were significantly lower (P < 0.05). Levels of albumin fragments and MMP-3 showed a significant correlation (P < 0.05). CONCLUSIONS: This study shows that proteomic techniques can be applied to investigate the involvement of proteolytic enzymes on the airways of mechanically ventilated premature infants.
Authors: R L Warner; L Beltran; E M Younkin; C S Lewis; S J Weiss; J Varani; K J Johnson Journal: Am J Respir Cell Mol Biol Date: 2001-05 Impact factor: 6.914
Authors: Jan Hirsch; Kirk C Hansen; Alma L Burlingame; Michael A Matthay Journal: Am J Physiol Lung Cell Mol Physiol Date: 2004-07 Impact factor: 5.464
Authors: D G Sweet; A E Curley; E Chesshyre; J Pizzotti; M S Wilbourn; H L Halliday; J A Warner Journal: Acta Paediatr Date: 2004-06 Impact factor: 2.299
Authors: Ikechukwu I Ekekezie; Donald W Thibeault; Stephen D Simon; Michael Norberg; Jeffrey D Merrill; Roberta A Ballard; Philip L Ballard; William E Truog Journal: Pediatrics Date: 2004-06 Impact factor: 7.124
Authors: Asma Ahmad; Charles L Cai; Dharmendra Kumar; Fayme Cai; Antoni D'Souza; Lawrence Fordjour; Taimur Ahmad; Gloria B Valencia; Jacob V Aranda; Kay D Beharry Journal: Am J Transl Res Date: 2014-10-11 Impact factor: 4.060
Authors: Massimo Antonelli; Elie Azoulay; Marc Bonten; Jean Chastre; Giuseppe Citerio; Giorgio Conti; Daniel De Backer; François Lemaire; Herwig Gerlach; Goran Hedenstierna; Michael Joannidis; Duncan Macrae; Jordi Mancebo; Salvatore M Maggiore; Alexandre Mebazaa; Jean-Charles Preiser; Jerôme Pugin; Jan Wernerman; Haibo Zhang Journal: Intensive Care Med Date: 2010-02-23 Impact factor: 17.440
Authors: Thomas Salaets; Jute Richter; Paul Brady; Julio Jimenez; Taro Nagatomo; Jan Deprest; Jaan Toelen Journal: PLoS One Date: 2015-08-28 Impact factor: 3.240