BACKGROUND AND OBJECTIVE: Porphyromonas gingivalis, a major periodontal pathogen, has been reported to be involved in atherogenesis. In order to further understand this pathogen's link with systemic inflammation and vascular disease, we investigated its influence on murine monocytes and macrophages from three different sources. MATERIAL AND METHODS: Concanavalin A-elicited peritoneal macrophages, peripheral blood monocyte-derived macrophages and WEHI 274.1 monocytes were infected with either P. gingivalis 381 or its non-invasive fimbriae-deficient mutant, DPG3. RESULTS: Infection with P. gingivalis 381 markedly induced monocyte migration and significantly enhanced production of the pro-inflammatory cytokines, tumor necrosis factor-alpha and interleukin-6. Consistent with a role for this pathogen's major fimbriae and/or its invasive capacity, infection with DPG3 had a minimal effect on both monocyte attraction and pro-inflammatory cytokine production. CONCLUSION: Since monocyte recruitment and activation are important steps in the development of vascular inflammation and atherosclerosis, these results suggest that P. gingivalis infection may be involved in these processes.
BACKGROUND AND OBJECTIVE:Porphyromonas gingivalis, a major periodontal pathogen, has been reported to be involved in atherogenesis. In order to further understand this pathogen's link with systemic inflammation and vascular disease, we investigated its influence on murine monocytes and macrophages from three different sources. MATERIAL AND METHODS: Concanavalin A-elicited peritoneal macrophages, peripheral blood monocyte-derived macrophages and WEHI 274.1 monocytes were infected with either P. gingivalis 381 or its non-invasive fimbriae-deficient mutant, DPG3. RESULTS:Infection with P. gingivalis 381 markedly induced monocyte migration and significantly enhanced production of the pro-inflammatory cytokines, tumor necrosis factor-alpha and interleukin-6. Consistent with a role for this pathogen's major fimbriae and/or its invasive capacity, infection with DPG3 had a minimal effect on both monocyte attraction and pro-inflammatory cytokine production. CONCLUSION: Since monocyte recruitment and activation are important steps in the development of vascular inflammation and atherosclerosis, these results suggest that P. gingivalis infection may be involved in these processes.
Authors: Nicole Delange; Suzanne Lindsay; Hector Lemus; Tracy L Finlayson; Scott T Kelley; Roberta A Gottlieb Journal: J Periodontol Date: 2018-02-23 Impact factor: 6.993
Authors: Benjamin Krämer; Moritz Kebschull; Michael Nowak; Ryan T Demmer; Manuela Haupt; Christian Körner; Sven Perner; Søren Jepsen; Jacob Nattermann; Panos N Papapanou Journal: Infect Immun Date: 2012-12-17 Impact factor: 3.441
Authors: Paulo H Rodrigues; Leticia Reyes; Amandeep S Chadda; Myriam Bélanger; Shannon M Wallet; Debra Akin; William Dunn; Ann Progulske-Fox Journal: PLoS One Date: 2012-12-26 Impact factor: 3.240
Authors: Moritz Kebschull; Manuela Haupt; Søren Jepsen; James Deschner; Georg Nickenig; Nikos Werner Journal: PLoS One Date: 2013-01-23 Impact factor: 3.240