BACKGROUND: Whether laparoscopic gastrectomy affects the number of gastric cancer cells exfoliated from the cancer-invaded serosa remains unclear. This study aimed to compare the detection rate of free gastric cancer cells in the peritoneal cavity during laparoscopic and open gastrectomy. METHODS: Intraoperative peritoneal washings were collected from 83 gastric cancer patients undergoing laparoscopic gastrectomy and 81 patients undergoing open surgery. Hematoxylin and eosin (H&E) staining and real-time reverse-transcription polymerase chain reaction (RT-PCR) were used to examine the free cancer cells. RESULTS: The postoperative positive rates of free cancer cells detected by cytological and real-time RT-PCR were 39.76 and 43.20% in the laparoscopic and open groups, respectively. Depth of tumor invasion, area of invaded serosa, regional lymph node involvement, and higher pathological staging were significantly associated with presence of free cancer cells. CONCLUSION: The laparoscopic techniques used in gastric cancer surgery did not increase the detection rate of free cancer cells in the peritoneal cavity compared with conventional techniques.
BACKGROUND: Whether laparoscopic gastrectomy affects the number of gastric cancer cells exfoliated from the cancer-invaded serosa remains unclear. This study aimed to compare the detection rate of free gastric cancer cells in the peritoneal cavity during laparoscopic and open gastrectomy. METHODS: Intraoperative peritoneal washings were collected from 83 gastric cancerpatients undergoing laparoscopic gastrectomy and 81 patients undergoing open surgery. Hematoxylin and eosin (H&E) staining and real-time reverse-transcription polymerase chain reaction (RT-PCR) were used to examine the free cancer cells. RESULTS: The postoperative positive rates of free cancer cells detected by cytological and real-time RT-PCR were 39.76 and 43.20% in the laparoscopic and open groups, respectively. Depth of tumor invasion, area of invaded serosa, regional lymph node involvement, and higher pathological staging were significantly associated with presence of free cancer cells. CONCLUSION: The laparoscopic techniques used in gastric cancer surgery did not increase the detection rate of free cancer cells in the peritoneal cavity compared with conventional techniques.
Authors: Francisco Tustumi; Wanderley Marques Bernardo; Andre Roncon Dias; Marcus Fernando Kodama Pertille Ramos; Ivan Cecconello; Bruno Zilberstein; Ulysses Ribeiro-Júnior Journal: Clinics (Sao Paulo) Date: 2016-12-01 Impact factor: 2.365
Authors: Mathieu Pecqueux; Johannes Fritzmann; Mariam Adamu; Kristian Thorlund; Christoph Kahlert; Christoph Reißfelder; Jürgen Weitz; Nuh N Rahbari Journal: Oncotarget Date: 2015-11-03