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Literature DB >> 1977694

Characterization of the class III region in different MHC haplotypes by pulsed-field gel electrophoresis.

I Dunham¹, C A Sargent, E Kendall, R D Campbell.

Abstract

The class III region of the human major histocompatibility complex (MHC) in seven HLA haplotypes has been analyzed using pulsed-field gel electrophoresis (PFGE), restriction enzymes that cut genomic DNA infrequently, and Southern blotting. In particular, extensive mapping with the enzyme Bss HII, which generates fragments in the size range 8-280 kilobases (kb), has revealed that in the haplotypes studied the DNA content of the class III region does not appear to vary other than as previously observed at the C4 and CYP21 loci.

Entities: Gene Species

Mesh：

Substances：
HLA Antigens

Year: 1990 PMID： 1977694 DOI： 10.1007/bf02114970

Source DB: PubMed Journal: Immunogenetics ISSN： 0093-7711 Impact factor: 2.846

37 in total

1. Human major histocompatibility complex contains a minimum of 19 genes between the complement cluster and HLA-B.

Authors: T Spies; M Bresnahan; J L Strominger
Journal: Proc Natl Acad Sci U S A Date: 1989-11 Impact factor: 11.205

Review 2. Polymorphism of the human complement component C4.

Authors: R D Campbell; I Dunham; E Kendall; C A Sargent
Journal: Exp Clin Immunogenet Date: 1990

3. CpG islands and HTF islands in the HLA class I region: investigation of the methylation status of class I genes leads to precise physical mapping of the HLA-B and -C genes.

Authors: P Pontarotti; G Chimini; C Nguyen; J Boretto; B R Jordan
Journal: Nucleic Acids Res Date: 1988-07-25 Impact factor: 16.971

4. Long-range restriction site mapping of mammalian genomic DNA.

Authors: W R Brown; A P Bird
Journal: Nature Date: 1986 Jul 31-Aug 6 Impact factor: 49.962

5. A model for the separation of large DNA molecules by crossed field gel electrophoresis.

Authors: E M Southern; R Anand; W R Brown; D S Fletcher
Journal: Nucleic Acids Res Date: 1987-08-11 Impact factor: 16.971

6. Structural organization of the DR subregion of the human major histocompatibility complex.

Authors: T Spies; R Sorrentino; J M Boss; K Okada; J L Strominger
Journal: Proc Natl Acad Sci U S A Date: 1985-08 Impact factor: 11.205

7. A new cluster of genes within the human major histocompatibility complex.

Authors: T Spies; G Blanck; M Bresnahan; J Sands; J L Strominger
Journal: Science Date: 1989-01-13 Impact factor: 47.728

8. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1984-02 Impact factor: 3.365

9. Mapping of steroid 21-hydroxylase genes adjacent to complement component C4 genes in HLA, the major histocompatibility complex in man.

Authors: M C Carroll; R D Campbell; R R Porter
Journal: Proc Natl Acad Sci U S A Date: 1985-01 Impact factor: 11.205

10. Extensive deletions and insertions in different MHC supratypes detected by pusled field gel electrophoresis.

Authors: K Tokunaga; G Saueracker; P H Kay; F T Christiansen; R Anand; R L Dawkins
Journal: J Exp Med Date: 1988-09-01 Impact factor: 14.307

8 in total

1. Genetic structure of the novel low-frequency haplotype HLA-B49, SC01, DR4 and its contribution to insulin-dependent diabetes susceptibility.

Authors: O G Segurado; P Iglesias-Casarrubios; P Morales; J Martínez-Laso; J Partanen; R D Campbell; A Arnaiz-Villena
Journal: Immunogenetics Date: 1992 Impact factor: 2.846

Characterization of the class III region in different MHC haplotypes by pulsed-field gel electrophoresis.

1. Human major histocompatibility complex contains a minimum of 19 genes between the complement cluster and HLA-B.

Review 2. Polymorphism of the human complement component C4.

3. CpG islands and HTF islands in the HLA class I region: investigation of the methylation status of class I genes leads to precise physical mapping of the HLA-B and -C genes.

4. Long-range restriction site mapping of mammalian genomic DNA.

5. A model for the separation of large DNA molecules by crossed field gel electrophoresis.

6. Structural organization of the DR subregion of the human major histocompatibility complex.

7. A new cluster of genes within the human major histocompatibility complex.

8. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

9. Mapping of steroid 21-hydroxylase genes adjacent to complement component C4 genes in HLA, the major histocompatibility complex in man.

10. Extensive deletions and insertions in different MHC supratypes detected by pusled field gel electrophoresis.

1. Genetic structure of the novel low-frequency haplotype HLA-B49, SC01, DR4 and its contribution to insulin-dependent diabetes susceptibility.

2. Molecular analysis of the MHC class II region in DR4, DR7, and DR9 haplotypes.

Review 3. G9a, a multipotent regulator of gene expression.

Review 4. "Both man & bird & beast": comparative organization of MHC genes.

5. Cloning of the HLA class II region in yeast artificial chromosomes.

6. Cloning and genomic characterization of LST1: a new gene in the human TNF region.

7. The G9a gene in the human major histocompatibility complex encodes a novel protein containing ankyrin-like repeats.

8. Evidence that gene G7a in the human major histocompatibility complex encodes valyl-tRNA synthetase.