BACKGROUND: Most cases of reduced vancomycin susceptibility in Staphylococcus aureus reported in the literature have been in methicillin-resistant strains. We report the development of reduced vancomycin susceptibility in a series of clonally related, methicillin-susceptible S. aureus (MSSA) clinical isolates. This isogenic series permitted us to determine whether the evolution of reduced vancomycin susceptibility in MSSA is similar to that seen in MRSA. METHODS: Differences in vancomycin population analysis profiles; chemical autolysis; vancomycin, oxacillin, and daptomycin minimum inhibitory concentrations; and bactericidal activities were examined. RESULTS: Progressive vancomycin resistance correlated with increasing daptomycin nonsusceptibility. Chemical autolysis and the bactericidal activity of vancomycin, oxacillin, and daptomycin were reduced in the final, vancomycin-intermediate S. aureus isolate, compared with the vancomycin-susceptible MSSA progenitor. CONCLUSIONS: Clinicians should recognize that reduced vancomycin susceptibility can occur in S. aureus irrespective of background methicillin susceptibility and that development of intermediate vancomycin susceptibility in MSSA may result in increased tolerance to several classes of anti-staphylococcal antibiotics.
BACKGROUND: Most cases of reduced vancomycin susceptibility in Staphylococcus aureus reported in the literature have been in methicillin-resistant strains. We report the development of reduced vancomycin susceptibility in a series of clonally related, methicillin-susceptible S. aureus (MSSA) clinical isolates. This isogenic series permitted us to determine whether the evolution of reduced vancomycin susceptibility in MSSA is similar to that seen in MRSA. METHODS: Differences in vancomycin population analysis profiles; chemical autolysis; vancomycin, oxacillin, and daptomycin minimum inhibitory concentrations; and bactericidal activities were examined. RESULTS: Progressive vancomycin resistance correlated with increasing daptomycin nonsusceptibility. Chemical autolysis and the bactericidal activity of vancomycin, oxacillin, and daptomycin were reduced in the final, vancomycin-intermediate S. aureus isolate, compared with the vancomycin-susceptible MSSA progenitor. CONCLUSIONS: Clinicians should recognize that reduced vancomycin susceptibility can occur in S. aureus irrespective of background methicillin susceptibility and that development of intermediate vancomycin susceptibility in MSSA may result in increased tolerance to several classes of anti-staphylococcal antibiotics.
Authors: Sean B Sullivan; Eloise D Austin; Stephania Stump; Barun Mathema; Susan Whittier; Franklin D Lowy; Anne-Catrin Uhlemann Journal: Antimicrob Agents Chemother Date: 2017-06-27 Impact factor: 5.191
Authors: Clare L Kinnear; Twisha S Patel; Carol L Young; Vincent Marshall; Duane W Newton; Andrew F Read; Robert J Woods Journal: Antimicrob Agents Chemother Date: 2019-03-27 Impact factor: 5.191
Authors: Kenneth V I Rolston; Weiqun Wang; Lior Nesher; Samuel A Shelburne; Randall A Prince Journal: J Antibiot (Tokyo) Date: 2015-12-02 Impact factor: 2.649
Authors: David R Cameron; Doyle V Ward; Xenia Kostoulias; Benjamin P Howden; Robert C Moellering; George M Eliopoulos; Anton Y Peleg Journal: J Infect Dis Date: 2012-04-05 Impact factor: 5.226
Authors: B J Werth; C Vidaillac; K P Murray; K L Newton; G Sakoulas; P Nonejuie; J Pogliano; M J Rybak Journal: Antimicrob Agents Chemother Date: 2013-02-19 Impact factor: 5.191
Authors: Stefanie Hebecker; Joern Krausze; Tatjana Hasenkampf; Julia Schneider; Maike Groenewold; Joachim Reichelt; Dieter Jahn; Dirk W Heinz; Jürgen Moser Journal: Proc Natl Acad Sci U S A Date: 2015-08-10 Impact factor: 11.205