Literature DB >> 19769456

Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi.

Vanina E Marquez1, Diego G Arias, Claudia V Piattoni, Carlos Robello, Alberto A Iglesias, Sergio A Guerrero.   

Abstract

Glutaredoxins play an important role in cellular functionality. A putative dithiol glutaredoxin is encoded in the genome of Trypanosoma cruzi. We cloned the gene and obtained the recombinant protein, which behaved as a typical thioltransferase. Activity was variable and dependent on the nature of reducer or oxidant agent used, or both. Epimastigote extracts exhibited similar activity, suggesting the occurrence of the protein in the parasite. Results support a redox scenario in T. cruzi, with glutaredoxin being involved mainly in reduction of glutathione disulfide as well as in deglutathionylation of target proteins.

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Year:  2010        PMID： 19769456     DOI： 10.1089/ars.2009.2907

Source DB:  PubMed          Journal:  Antioxid Redox Signal        ISSN： 1523-0864            Impact factor:   8.401


  7 in total

Review 1.  Redox metabolism in mitochondria of trypanosomatids.

Authors:  Ana M Tomás; Helena Castro
Journal:  Antioxid Redox Signal       Date:  2012-11-15       Impact factor: 8.401

Review 2.  Mono- and dithiol glutaredoxins in the trypanothione-based redox metabolism of pathogenic trypanosomes.

Authors:  Marcelo A Comini; R Luise Krauth-Siegel; Massimo Bellanda
Journal:  Antioxid Redox Signal       Date:  2012-10-25       Impact factor: 8.401

3.  The dithiol glutaredoxins of african trypanosomes have distinct roles and are closely linked to the unique trypanothione metabolism.

Authors:  Sevgi Ceylan; Vera Seidel; Nicole Ziebart; Carsten Berndt; Natalie Dirdjaja; R Luise Krauth-Siegel
Journal:  J Biol Chem       Date:  2010-09-08       Impact factor: 5.157

Review 4.  Thioredoxins, glutaredoxins, and peroxiredoxins--molecular mechanisms and health significance: from cofactors to antioxidants to redox signaling.

Authors:  Eva-Maria Hanschmann; José Rodrigo Godoy; Carsten Berndt; Christoph Hudemann; Christopher Horst Lillig
Journal:  Antioxid Redox Signal       Date:  2013-03-28       Impact factor: 8.401

5.  Cloning, expression, purification, and characterization of glutaredoxin from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178.

Authors:  Quanfu Wang; Yanhua Hou; Yonglei Shi; Xiao Han; Qian Chen; Zhiguo Hu; Yuanping Liu; YuJin Li
Journal:  Biomed Res Int       Date:  2014-07-07       Impact factor: 3.411

6.  Molecular characterization and serodiagnostic potential of a novel dithiol glutaredoxin 1 from Echinococcus granulosus.

Authors:  Xingju Song; Min Yan; Dandan Hu; Yu Wang; Ning Wang; Xiaobin Gu; Xuerong Peng; Guangyou Yang
Journal:  Parasit Vectors       Date:  2016-08-17       Impact factor: 3.876

7.  A glutaredoxin in the mitochondrial intermembrane space has stage-specific functions in the thermo-tolerance and proliferation of African trypanosomes.

Authors:  Samantha Ebersoll; Blessing Musunda; Torsten Schmenger; Natalie Dirdjaja; Mariana Bonilla; Bruno Manta; Kathrin Ulrich; Marcelo A Comini; R Luise Krauth-Siegel
Journal:  Redox Biol       Date:  2018-01-31       Impact factor: 11.799
  7 in total

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