BACKGROUND: Older studies of diabetes development typically utilized a 7-day incubation polyethylene glycol competitive insulin autoantibody assay (CIAA). Our standard micro-IAA assay (mIAA) utilizes precipitation with proteins A/G and 1-day incubation (1-day mIAA), but is less sensitive compared to the CIAA assay. METHODS: We performed CIAA and mIAA assays in various conditions. We analyzed serum samples from 446 type 1 diabetes patients, from another set of 247 type 1 diabetes patients within 2 weeks of initiation of insulin treatment, from 150 healthy control donors, from 22 healthy participants in the diabetes autoimmunity study in the young (DAISY), and also coded sera from 50 patients with newly diagnosed type 1 diabetes and 50 blood donor control samples. RESULTS: In the process of our study, we found that the key condition was the incubation time. Therefore, we extended the incubation time to 7 days (7-day mIAA assay). No CIAA-negative control was positive with either 1-day or 7-day mIAA. In a new onset type 1 diabetes and at risk cohorts (DAISY study), the 7-day mIAA identified an additional 18% as being positive along with 16% of those who were initially 1-day mIAA negative and CIAA positive. Most subjects detectable only with the 7-day mIAA assay had intermediate levels of CIAA (80-300 nU/mL) (p = 0.01). CONCLUSIONS: The 7-day mIAA assay identifies a small but significant additional subset of individuals positive on the CIAA assay, while preserving specificity. (c) 2009 John Wiley & Sons, Ltd.
BACKGROUND: Older studies of diabetes development typically utilized a 7-day incubation polyethylene glycol competitive insulin autoantibody assay (CIAA). Our standard micro-IAA assay (mIAA) utilizes precipitation with proteins A/G and 1-day incubation (1-day mIAA), but is less sensitive compared to the CIAA assay. METHODS: We performed CIAA and mIAA assays in various conditions. We analyzed serum samples from 446 type 1 diabetespatients, from another set of 247 type 1 diabetespatients within 2 weeks of initiation of insulin treatment, from 150 healthy control donors, from 22 healthy participants in the diabetes autoimmunity study in the young (DAISY), and also coded sera from 50 patients with newly diagnosed type 1 diabetes and 50 blood donor control samples. RESULTS: In the process of our study, we found that the key condition was the incubation time. Therefore, we extended the incubation time to 7 days (7-day mIAA assay). No CIAA-negative control was positive with either 1-day or 7-day mIAA. In a new onset type 1 diabetes and at risk cohorts (DAISY study), the 7-day mIAA identified an additional 18% as being positive along with 16% of those who were initially 1-day mIAA negative and CIAA positive. Most subjects detectable only with the 7-day mIAA assay had intermediate levels of CIAA (80-300 nU/mL) (p = 0.01). CONCLUSIONS: The 7-day mIAA assay identifies a small but significant additional subset of individuals positive on the CIAA assay, while preserving specificity. (c) 2009 John Wiley & Sons, Ltd.
Authors: Ashok Sharma; Xiang Liu; David Hadley; William Hagopian; Wei-Min Chen; Suna Onengut-Gumuscu; Carina Törn; Andrea K Steck; Brigitte I Frohnert; Marian Rewers; Anette-G Ziegler; Åke Lernmark; Jorma Toppari; Jeffrey P Krischer; Beena Akolkar; Stephen S Rich; Jin-Xiong She Journal: J Autoimmun Date: 2018-01-05 Impact factor: 14.511
Authors: Janet K Snell-Bergeon; Jennifer Smith; Fran Dong; Anna E Barón; Kathy Barriga; Jill M Norris; Marian Rewers Journal: Diabetes Care Date: 2012-10-05 Impact factor: 19.112
Authors: Carina Törn; David Hadley; Hye-Seung Lee; William Hagopian; Åke Lernmark; Olli Simell; Marian Rewers; Anette Ziegler; Desmond Schatz; Beena Akolkar; Suna Onengut-Gumuscu; Wei-Min Chen; Jorma Toppari; Juha Mykkänen; Jorma Ilonen; Stephen S Rich; Jin-Xiong She; Andrea K Steck; Jeffrey Krischer Journal: Diabetes Date: 2014-11-24 Impact factor: 9.337