| Literature DB >> 19767417 |
Gary Temple, Daniela S Gerhard, Rebekah Rasooly, Elise A Feingold, Peter J Good, Cristen Robinson, Allison Mandich, Jeffrey G Derge, Jeanne Lewis, Debonny Shoaf, Francis S Collins, Wonhee Jang, Lukas Wagner, Carolyn M Shenmen, Leonie Misquitta, Carl F Schaefer, Kenneth H Buetow, Tom I Bonner, Linda Yankie, Ming Ward, Lon Phan, Alex Astashyn, Garth Brown, Catherine Farrell, Jennifer Hart, Melissa Landrum, Bonnie L Maidak, Michael Murphy, Terence Murphy, Bhanu Rajput, Lillian Riddick, David Webb, Janet Weber, Wendy Wu, Kim D Pruitt, Donna Maglott, Adam Siepel, Brona Brejova, Mark Diekhans, Rachel Harte, Robert Baertsch, Jim Kent, David Haussler, Michael Brent, Laura Langton, Charles L G Comstock, Michael Stevens, Chaochun Wei, Marijke J van Baren, Kourosh Salehi-Ashtiani, Ryan R Murray, Lila Ghamsari, Elizabeth Mello, Chenwei Lin, Christa Pennacchio, Kirsten Schreiber, Nicole Shapiro, Amber Marsh, Elizabeth Pardes, Troy Moore, Anita Lebeau, Mike Muratet, Blake Simmons, David Kloske, Stephanie Sieja, James Hudson, Praveen Sethupathy, Michael Brownstein, Narayan Bhat, Joseph Lazar, Howard Jacob, Chris E Gruber, Mark R Smith, John McPherson, Angela M Garcia, Preethi H Gunaratne, Jiaqian Wu, Donna Muzny, Richard A Gibbs, Alice C Young, Gerard G Bouffard, Robert W Blakesley, Jim Mullikin, Eric D Green, Mark C Dickson, Alex C Rodriguez, Jane Grimwood, Jeremy Schmutz, Richard M Myers, Martin Hirst, Thomas Zeng, Kane Tse, Michelle Moksa, Merinda Deng, Kevin Ma, Diana Mah, Johnson Pang, Greg Taylor, Eric Chuah, Athena Deng, Keith Fichter, Anne Go, Stephanie Lee, Jing Wang, Malachi Griffith, Ryan Morin, Richard A Moore, Michael Mayo, Sarah Munro, Susan Wagner, Steven J M Jones, Robert A Holt, Marco A Marra, Sun Lu, Shuwei Yang, James Hartigan, Marcus Graf, Ralf Wagner, Stanley Letovksy, Jacqueline C Pulido, Keith Robison, Dominic Esposito, James Hartley, Vanessa E Wall, Ralph F Hopkins, Osamu Ohara, Stefan Wiemann.
Abstract
Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.Entities:
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Year: 2009 PMID： 19767417 PMCID： PMC2792178 DOI： 10.1101/gr.095976.109
Source DB: PubMed Journal: Genome Res ISSN： 1088-9051 Impact factor: 9.043