WeiJie Liu1, Kai Wang, BaoZhen Li, HongLi Yuan, JinShui Yang. 1. State Key Lab for Agrobiotechnology, Center of Biomass Engineering, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Abstract
A novel intracellular bioflocculant (named MBF-W6) produced by Chryseobacterium daeguense W6 cultured in low nutrition medium was investigated in this study. The effects of carbon source, nitrogen source, C/N ratio, initial pH, inoculum size, culture temperature and shaking speed on MBF-W6 production were studied. Chemical analysis showed that the purified MBF-W6 was mainly composed of 32.4% protein, 13.1% polysaccharide and 6.8% nucleic acid. Fourier-transform infrared (FTIR) spectroscopy indicated the presence of carboxyl, hydroxyl, and methoxyl groups. The elemental analysis of purified MBF-W6 revealed that the mass proportion of C, H, O, N and S was 40.92:6.53:44.01:8.53:1.01 (w/w) correspondingly. MBF-W6 had good flocculating rate in Kaolin suspension without any cation addition. The highest flocculating rate of 96.9% was achieved under the optimal conditions (bioflocculant dosage 1.2 mg l(-1), pH 5.6 and temperature 15 degrees C).
A novel intracellular bioflocculant (named MBF-W6) produced by Chryseobacterium daeguense W6 cultured in low nutrition medium was investigated in this study. The effects of n class="Chemical">carbon source, nitrogen source, C/N ratio, initial pH, inoculum size, culture temperature and shaking speed on MBF-W6 production were studied. Chemical analysis showed that the purified MBF-W6 was mainly composed of 32.4% protein, 13.1% polysaccharide and 6.8% nucleic acid. Fourier-transform infrared (FTIR) spectroscopy indicated the presence of carboxyl, hydroxyl, and methoxyl groups. The elemental analysis of purified MBF-W6 revealed that the mass proportion of C, H, O, N and S was 40.92:6.53:44.01:8.53:1.01 (w/w) correspondingly. MBF-W6 had good flocculating rate in Kaolin suspension without any cation addition. The highest flocculating rate of 96.9% was achieved under the optimal conditions (bioflocculant dosage 1.2 mg l(-1), pH 5.6 and temperature 15 degrees C).
Authors: Anthony Ugbenyen; Sekelwa Cosa; Leonard Mabinya; Olubukola O Babalola; Farhad Aghdasi; Anthony Okoh Journal: Int J Environ Res Public Health Date: 2012-06-04 Impact factor: 3.390
Authors: Kunle Okaiyeto; Uchechukwu U Nwodo; Leonard V Mabinya; Arinze S Okoli; Anthony I Okoh Journal: Int J Mol Sci Date: 2015-06-08 Impact factor: 5.923
Authors: Kunle Okaiyeto; Uchechukwu U Nwodo; Leonard V Mabinya; Anthony I Okoh Journal: Int J Environ Res Public Health Date: 2013-10-16 Impact factor: 3.390