Distinct roles for NtrC and GlnK in nitrogen regulation of the Pseudomonas sp. strain ADP cyanuric acid utilization operon.

The Pseudomonas sp. strain ADP atzDEF operon encodes the enzymes involved in cyanuric acid mineralization, the final stage of the s-triazine herbicide atrazine degradative pathway. We have previously shown that atzDEF is under nitrogen control in both its natural host and Pseudomonas putida KT2442. Expression of atzDEF requires the divergently encoded LysR-type transcriptional regulator AtzR. Here, we take advantage of the poor induction of atzDEF in Escherichia coli to identify Pseudomonas factors involved in nitrogen control of atzDEF expression. Simultaneous production of P. putida NtrC and GlnK, along with AtzR, restored the normal atzDEF regulatory pattern. Gene expression analysis in E. coli and P. putida indicated that NtrC activates atzR expression, while the role of GlnK is to promote AtzR activation of atzDEF under nitrogen limitation. Activation of atzDEF in a mutant background deficient in GlnK uridylylation suggests that post-translational modification is not strictly required for transduction of the nitrogen limitation signal to AtzR. The present data and our previous results are integrated in a regulatory circuit that describes all the known responses of the atzDEF operon.