[Laboratory study on the relationship between drinking water and hepatoma--quantitative evaluation using hepatic gamma-GT method].

One to two-month-old male Fischer 344 (F-344) rats were used in this investigation. The experiment was devised simulating the Two-stage Hepatocarcinogenesis Model described by Pitot. Quantitative stereological method was applied to evaluate the gamma-GT enzyme altered foci (EAF) in examined liver slides. The results showed that pond-ditch water had an enhancement effect on the expression of gamma-GT in the livers of male F-344 rats, where as well water had no such effect. When AFB1 was given alone to the male F-344 rats after partial hepatectomy, there was no effect on gamma-GT, if it was followed by pond-ditch water the rats would express high frequency of EAF. In this experiment the effect of organochlorinated pesticide DDT on hepatic gamma-GT was also studied. It showed a strong influence on the quantity and size of EAF.