Literature DB >> 19763295

A new assay format for NF-kappaB based on a DNA triple helix and a fluorescence resonance energy transfer.

Dominik Altevogt1, Andrea Hrenn, Claudia Kern, Lilia Clima, Willi Bannwarth, Irmgard Merfort.   

Abstract

Herein we report a feasibility study for a new concept to detect DNA binding protein NF-kappaB based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.

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Year:  2009        PMID： 19763295     DOI： 10.1039/b906447h

Source DB:  PubMed          Journal:  Org Biomol Chem        ISSN： 1477-0520            Impact factor:   3.876


  3 in total

1.  Hairpin-like fluorescent probe for imaging of NF-κB transcription factor activity.

Authors:  Valeri Metelev; Surong Zhang; David Tabatadze; Alexei Bogdanov
Journal:  Bioconjug Chem       Date:  2011-03-21       Impact factor: 4.774

2.  Sensing of transcription factor binding via cyanine dye pair fluorescence lifetime changes.

Authors:  Alexei A Bogdanov; Valeriy Metelev; Surong Zhang; Anand T N Kumar
Journal:  Mol Biosyst       Date:  2012-06-19

3.  A rapid and high-throughput quantitation assay of the nuclear factor κB activity using fluorescence correlation spectroscopy in the setting of clinical laboratories.

Authors:  Kenu Harada; Shintaro Mikuni; Hideyuki Beppu; Hideki Niimi; Shigeki Abe; Nobuko Hano; Koichi Yamagata; Masataka Kinjo; Isao Kitajima
Journal:  PLoS One       Date:  2013-10-04       Impact factor: 3.240
  3 in total

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