Literature DB >> 19763290

Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases.

Yajun Yan1, Chia-Chi Lee, James C Liao.   

Abstract

We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).

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Year:  2009        PMID： 19763290     DOI： 10.1039/b913501d

Source DB:  PubMed          Journal:  Org Biomol Chem        ISSN： 1477-0520            Impact factor:   3.876


  28 in total

1.  Enhanced production of (R,R)-2,3-butanediol by metabolically engineered Klebsiella oxytoca.

Authors:  Jong Myoung Park; Chelladurai Rathnasingh; Hyohak Song
Journal:  J Ind Microbiol Biotechnol       Date:  2015-08-15       Impact factor: 3.346

2.  Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production.

Authors:  Fangmin Bai; Lu Dai; Jiying Fan; Ngoctu Truong; Ben Rao; Liaoyuan Zhang; Yaling Shen
Journal:  J Ind Microbiol Biotechnol       Date:  2015-02-10       Impact factor: 3.346

3.  Establishing synthesis pathway-host compatibility via enzyme solubility.

Authors:  Sara A Amin; Venkatesh Endalur Gopinarayanan; Nikhil U Nair; Soha Hassoun
Journal:  Biotechnol Bioeng       Date:  2019-03-29       Impact factor: 4.530

4.  Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel NADPH-dependent primary-secondary alcohol dehydrogenase.

Authors:  Michael Köpke; Monica L Gerth; Danielle J Maddock; Alexander P Mueller; FungMin Liew; Séan D Simpson; Wayne M Patrick
Journal:  Appl Environ Microbiol       Date:  2014-03-21       Impact factor: 4.792

5.  Novel (2R,3R)-2,3-butanediol dehydrogenase from potential industrial strain Paenibacillus polymyxa ATCC 12321.

Authors:  Bo Yu; Jibin Sun; Rajesh Reddy Bommareddy; Lifu Song; An-Ping Zeng
Journal:  Appl Environ Microbiol       Date:  2011-04-29       Impact factor: 4.792

6.  Cloning, expression and characterization of glycerol dehydrogenase involved in 2,3-butanediol formation in Serratia marcescens H30.

Authors:  Liaoyuan Zhang; Quanming Xu; Xiaoqian Peng; Boheng Xu; Yuehao Wu; Yulong Yang; Shujing Sun; Kaihui Hu; Yaling Shen
Journal:  J Ind Microbiol Biotechnol       Date:  2014-07-01       Impact factor: 3.346

7.  Molecular characterization of an NADPH-dependent acetoin reductase/2,3-butanediol dehydrogenase from Clostridium beijerinckii NCIMB 8052.

Authors:  John Raedts; Marco A J Siemerink; Mark Levisson; John van der Oost; Servé W M Kengen
Journal:  Appl Environ Microbiol       Date:  2014-01-17       Impact factor: 4.792

8.  Enantioselective Synthesis of Vicinal (R,R)-Diols by Saccharomyces cerevisiae Butanediol Dehydrogenase.

Authors:  Eduard Calam; Eva González-Roca; M Rosario Fernández; Sylvie Dequin; Xavier Parés; Albert Virgili; Josep A Biosca
Journal:  Appl Environ Microbiol       Date:  2016-01-04       Impact factor: 4.792

9.  Engineering cofactor flexibility enhanced 2,3-butanediol production in Escherichia coli.

Authors:  Keming Liang; Claire R Shen
Journal:  J Ind Microbiol Biotechnol       Date:  2017-11-07       Impact factor: 3.346

10.  Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high-level production of L-2,3-butanediol.

Authors:  Jang Min Park; Won-Kyung Hong; Sung-Mok Lee; Sun-Yeon Heo; You Ree Jung; In Yeong Kang; Baek-Rock Oh; Jeong-Woo Seo; Chul Ho Kim
Journal:  J Ind Microbiol Biotechnol       Date:  2014-07-19       Impact factor: 3.346
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