Literature DB >> 1975800

HLA-DQ RFLP variants of five HLA-DQw2-bearing major histocompatibility complex extended haplotypes.

J J Yunis1, M Wescott, S J Lechin, S M Alosco, Z Awdeh, C A Alper, E J Yunis.   

Abstract

We have analyzed genomic DNA in a large number of independent examples of five HLA-DQw2-bearing extended haplotypes for their associated subtypes by restriction fragment length polymorphism (RFLP) using DRB, DQA, and DQB probes after Taq I and Pst I digestion and Southern blotting. In addition to three previously described HLA-DQw2 subtypes, DQw2a, DQw2b, and DQw2c, we observed a fourth subtype, HLA-DQw2d, characterized by 5.8 kilobase (kb) DRB/Taq I, 2.4, 2.3, and 1.8 kb DQB/Taq I, and 8.0 and 2.3 kb DQA/Pst I fragments. All 22 independent examples of the extended haplotype [HLA-B8, SC01, DR3] carried DQw2a and all 11 independent examples of [HLA-B18, F1C30, DR3] carried DQw2b. In addition, all independent examples (21 and 4, respectively) of two DR7-carrying extended haplotypes, [HLA-B44, FC31, DR7] and [HLA-Bw47, FC91,0,DR7], carried DQw2c and all independent examples of [HLA-Bw57, SC61, DR7] carried DQw2d. Our results show that the DNA in the DR/DQ region of extended haplotypes is relatively fixed and that different DQw2 subtypes characterize different DQw2-bearing extended haplotypes.

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Year:  1990        PMID： 1975800     DOI： 10.1007/bf00210445

Source DB:  PubMed          Journal:  Immunogenetics        ISSN： 0093-7711            Impact factor:   2.846


  14 in total

1.  Comparison of membrane proteins of Burkitt's lymphoma and EBV-transformed B lymphoblast cell lines and of Con A-activated T lymphocytes and T lymphoblast cell lines.

Authors:  R C Spiro; J L DeMartino; W Boto; H Lazarus; R E Humphreys
Journal:  Leuk Res       Date:  1979       Impact factor: 3.156

2.  HLA class II RFLPs are haplotype-specific.

Authors:  S W Serjeantson; M R Kohonen-Corish; H Dunckley; M A Reid
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1986

3.  Molecular analysis distinguishes two HLA-DR3-bearing major histocompatibility complex extended haplotypes.

Authors:  M Z Wescott; Z L Awdeh; E J Yunis; C A Alper
Journal:  Immunogenetics       Date:  1987       Impact factor: 2.846

4.  "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365

5.  The major histocompatibility complex in man.

Authors:  J Dausset
Journal:  Science       Date:  1981-09-25       Impact factor: 47.728

6.  Extended major histocompatibility complex haplotypes in man: role of alleles analogous to murine t mutants.

Authors:  C A Alper; Z L Awdeh; D D Raum; E J Yunis
Journal:  Clin Immunol Immunopathol       Date:  1982-08

7.  Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay.

Authors:  E O Long; C T Wake; M Strubin; N Gross; R S Accolla; S Carrel; B Mach
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

8.  cDNA clone for the heavy chain of the human B cell alloantigen DC1: strong sequence homology to the HLA-DR heavy chain.

Authors:  C Auffray; A J Korman; M Roux-Dosseto; R Bono; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

9.  A DR3-related DX alpha gene polymorphism strongly associates with insulin-dependent diabetes mellitus.

Authors:  G A Hitman; J Sachs; P Cassell; J Awad; G F Bottazzo; A C Tarn; G Schwartz; J P Monson; H Festenstein
Journal:  Immunogenetics       Date:  1986       Impact factor: 2.846

Review 10.  Possible human analogs of the murine T/t complex.

Authors:  C A Alper; Z L Awdeh; D D Raum; E J Yunis
Journal:  Exp Clin Immunogenet       Date:  1985
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