OBJECTIVE: The loss of podocytes has been reported to have a role in the onset and progression of diabetic nephropathy (DN). Although structural changes such as podocyte hypertrophy are considered to be associated with podocyte loss, the relationship has not been thoroughly investigated using human DN renal tissues. METHODS: The subjects were 17 patients with DN diagnosed histopathologically by renal biopsy. Immunostaining was performed with antibodies for Wilm's tumor 1 (WT1) and synaptopodin (SPD), which are markers of podocytes, to determine the number of podocytes and assess podocyte hypertrophy. RESULTS: The number of podocytes was decreased in DN patients compared with the controls. An inverse correlation was observed between the number of podocytes and both the urinary protein excretion and the extent of mesangial expansion. Podocyte hypertrophy was also more marked in DN patients compared with controls. CONCLUSION: Based on these results, podocyte loss and hypertrophy were suggested to be involved in the development and progression of human DN.
OBJECTIVE: The loss of podocytes has been reported to have a role in the onset and progression of diabetic nephropathy (DN). Although structural changes such as podocyte hypertrophy are considered to be associated with podocyte loss, the relationship has not been thoroughly investigated using human DN renal tissues. METHODS: The subjects were 17 patients with DN diagnosed histopathologically by renal biopsy. Immunostaining was performed with antibodies for Wilm's tumor 1 (WT1) and synaptopodin (SPD), which are markers of podocytes, to determine the number of podocytes and assess podocyte hypertrophy. RESULTS: The number of podocytes was decreased in DN patients compared with the controls. An inverse correlation was observed between the number of podocytes and both the urinary protein excretion and the extent of mesangial expansion. Podocyte hypertrophy was also more marked in DN patients compared with controls. CONCLUSION: Based on these results, podocyte loss and hypertrophy were suggested to be involved in the development and progression of human DN.
Authors: J Funk; V Ott; A Herrmann; W Rapp; S Raab; W Riboulet; A Vandjour; E Hainaut; A Benardeau; T Singer; B Jacobsen Journal: Histochem Cell Biol Date: 2015-12-15 Impact factor: 4.304
Authors: Darshana Govind; Jan U Becker; Jeffrey Miecznikowski; Avi Z Rosenberg; Julien Dang; Pierre Louis Tharaux; Rabi Yacoub; Friedrich Thaiss; Peter F Hoyer; David Manthey; Brendon Lutnick; Amber M Worral; Imtiaz Mohammad; Vighnesh Walavalkar; John E Tomaszewski; Kuang-Yu Jen; Pinaki Sarder Journal: J Am Soc Nephrol Date: 2021-09-03 Impact factor: 10.121
Authors: E Jennifer Weil; Kevin V Lemley; Clinton C Mason; Berne Yee; Lois I Jones; Kristina Blouch; Tracy Lovato; Meghan Richardson; Bryan D Myers; Robert G Nelson Journal: Kidney Int Date: 2012-06-20 Impact factor: 10.612