BACKGROUND: The presence of granuloma, visualized in histopathology for diagnosing tuberculosis in tissue samples, is not a specific finding. Moreover, histopathological examination of tissue sections needs one to two weeks for final reporting. A rapid and sensitive method is therefore needed for detection of Mycobacterium tuberculosis in these paucibacillary tissue samples. METHODOLOGY: A PCR-assay specific for IS6110 was evaluated for 104 different tissue samples in comparison to histopathology that was considered gold standard. RESULTS: PCR showed 74.1% sensitivity and 96.1% specificity. False positive and false negative results were observed in three (2.88%) and seven (6.73%) samples, respectively. Positive agreement between histopathology and PCR was observed as 0.737, indicating substantial good agreement between two tests. CONCLUSIONS: PCR can be used for early diagnosis of tuberculosis in tissue samples that can help to initiate timely anti-tubercular treatment and prevent progression to irreversible changes.
BACKGROUND: The presence of granuloma, visualized in histopathology for diagnosing tuberculosis in tissue samples, is not a specific finding. Moreover, histopathological examination of tissue sections needs one to two weeks for final reporting. A rapid and sensitive method is therefore needed for detection of Mycobacterium tuberculosis in these paucibacillary tissue samples. METHODOLOGY: A PCR-assay specific for IS6110 was evaluated for 104 different tissue samples in comparison to histopathology that was considered gold standard. RESULTS: PCR showed 74.1% sensitivity and 96.1% specificity. False positive and false negative results were observed in three (2.88%) and seven (6.73%) samples, respectively. Positive agreement between histopathology and PCR was observed as 0.737, indicating substantial good agreement between two tests. CONCLUSIONS: PCR can be used for early diagnosis of tuberculosis in tissue samples that can help to initiate timely anti-tubercular treatment and prevent progression to irreversible changes.
Authors: Preetjote Gill; Nicholas R Coatsworth; Justin S Gundara; Thomas J Hugh; Jaswinder S Samra Journal: World J Surg Date: 2013-05 Impact factor: 3.352
Authors: Hicham ELmajdoubi; Mariam El Yahiyaoui; Marouane Baiss; Mohamed Bouzroud; El Mehdi Aboulfeth; Mohammed Najih; Sidi Mohamed Bouchentouf; Hakim ELkaoui; Ahmed Bounaim Journal: Case Rep Surg Date: 2021-06-30
Authors: Angelika Agdestein; Tone B Johansen; Vladimir Polaček; Bjørn Lium; Gudmund Holstad; Dejan Vidanović; Sanja Aleksić-Kovačević; Anne Jørgensen; Jonas Žultauskas; Sigrun F Nilsen; Berit Djønne Journal: BMC Vet Res Date: 2011-10-21 Impact factor: 2.741