Literature DB >> 19751775

Flow cytometric analysis of benthic prokaryotes attached to sediment particles.

Stefano Amalfitano1, Stefano Fazi Alberto Puddu.   

Abstract

Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity.

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Year:  2009        PMID： 19751775     DOI： 10.1016/j.mimet.2009.09.005

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

1.  Benthic Microbial Communities in a Seasonally Ice-Covered Sub-Arctic River (Pasvik River, Norway) Are Shaped by Site-Specific Environmental Conditions.

Authors:  Maria Papale; Carmen Rizzo; Stefania Giannarelli; Gabriella Caruso; Stefano Amalfitano; Paul Eric Aspholm; Giovanna Maimone; Stefano Miserocchi; Alessandro Ciro Rappazzo; Angelina Lo Giudice; Maurizio Azzaro
Journal:  Microorganisms       Date:  2022-05-12

2.  Optimization of a Method To Quantify Soil Bacterial Abundance by Flow Cytometry.

Authors:  Banafshe Khalili; Claudia Weihe; Sarah Kimball; Katharina T Schmidt; Jennifer B H Martiny
Journal:  mSphere       Date:  2019-10-09       Impact factor: 4.389

3.  An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry.

Authors:  Mara Elena Heinrichs; Daniele De Corte; Bert Engelen; Donald Pan
Journal:  Viruses       Date:  2021-01-13       Impact factor: 5.048

4.  Flow Cytometric Assessment of Bacterial Abundance in Soils, Sediments and Sludge.

Authors:  Aline Frossard; Frederik Hammes; Mark O Gessner
Journal:  Front Microbiol       Date:  2016-06-14       Impact factor: 5.640
  4 in total

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