Literature DB >> 19748084

Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm.

Rebeca Santiso1, María Tamayo, Jaime Gosálvez, Marcos Meseguer, Nicolás Garrido, José Luis Fernández.   

Abstract

Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8-oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage. In all samples examined, increased levels of 8-oxoguanine were present only in those spermatozoa with fragmented DNA, suggesting a link between both DNA damage types. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

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Year:  2009        PMID： 19748084     DOI： 10.1016/j.fertnstert.2009.07.969

Source DB:  PubMed          Journal:  Fertil Steril        ISSN： 0015-0282            Impact factor:   7.329


  11 in total

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2.  The quality of sperm preparation medium affects the motility, viability, and DNA integrity of human spermatozoa.

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3.  Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in In Vitro Fertilization and Intracytoplasmic Sperm Injection.

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4.  Sperm DNA damage compromises embryo development, but not oocyte fertilisation in pigs.

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5.  Dysregulation of the Acrosome Formation Network by 8-oxoguanine (8-oxoG) in Infertile Sperm: A Case Report with Advanced Techniques.

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6.  In subfertile couple, abdominal fat loss in men is associated with improvement of sperm quality and pregnancy: a case-series.

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Review 7.  Oxidative stress and male reproductive health.

Authors:  Robert J Aitken; Tegan B Smith; Matthew S Jobling; Mark A Baker; Geoffry N De Iuliis
Journal:  Asian J Androl       Date:  2014 Jan-Feb       Impact factor: 3.285

8.  Inverse correlation between reactive oxygen species in unwashed semen and sperm motion parameters as measured by a computer-assisted semen analyzer.

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Review 9.  Single and Double Strand Sperm DNA Damage: Different Reproductive Effects on Male Fertility.

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Journal:  Genes (Basel)       Date:  2019-01-31       Impact factor: 4.096

10.  Use of a male antioxidant nutraceutical is associated with superior live birth rates during IVF treatment.

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