The Bv8 gene from Bombina orientalis: molecular cloning, genomic organization and functional characterization of the promoter.

Bv8 is a secreted peptide from Bombina variegata skin glands with a molecular mass close to 8kDa that is conserved in fish, amphibians and mammals. Bv8 has diverse regulatory roles, including an involvement in hematopoiesis and immunomodulation. Here we report the genomic organization of the gene from Bombina orientalis coding for the Bv8 homolog (Bo8). It contains three exons separated by two large introns. Several putative transcription factor binding sites have been identified in the promoter sequence. Functional analysis of this region was performed using a yeast genetic system. The results indicate that the transcription factors AP-1, NF-kappaB and NFAT are involved in the regulation of the expression of Bo8. Hence, amphibians are a useful model for the study of transcriptional regulation of all Bv8 homologs.