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Literature DB >> 19747823

Synthetic substrate for application in both high and low throughput assays for botulinum neurotoxin B protease inhibitors.

Nicholas T Salzameda¹, Joseph T Barbieri, Kim D Janda.

Abstract

A FRET peptide substrate was synthesized and evaluated for enzymatic cleavage by the BoNT/B light chain protease. The FRET substrate was found to be useful in both a high throughput assay to uncover initial 'hits' and a low throughput HPLC assay to determine kinetic parameters and modes of inhibition.

Entities: Chemical Disease Gene Mutation Species

Mesh：

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Year: 2009 PMID： 19747823 PMCID： PMC2753755 DOI： 10.1016/j.bmcl.2009.08.079

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

27 in total

1. High-throughput assays for botulinum neurotoxin proteolytic activity: serotypes A, B, D, and F.

Authors: J J Schmidt; R G Stafford; C B Millard
Journal: Anal Biochem Date: 2001-09-01 Impact factor: 3.365

2. A capillary electrophoresis technique for evaluating botulinum neurotoxin B light chain activity.

Authors: Michael Adler; Harlan F Shafer; Heather A Manley; Brennie E Hackley; James D Nicholson; James E Keller; Michael C Goodnough
Journal: J Protein Chem Date: 2003-07

3. Rapid microplate assay for monitoring botulinum neurotoxin B catalytic activity.

Authors: J E Keller; J L Nowakowski; M G Filbert; M Adler
Journal: J Appl Toxicol Date: 1999-12 Impact factor: 3.446

4. Synthesis, characterization and development of a high-throughput methodology for the discovery of botulinum neurotoxin a inhibitors.

Authors: Grant E Boldt; Jack P Kennedy; Mark S Hixon; Laura A McAllister; Joseph T Barbieri; Saul Tzipori; Kim D Janda
Journal: J Comb Chem Date: 2006 Jul-Aug

5. Development of potent inhibitors of botulinum neurotoxin type B.

Authors: Christine Anne; Serge Turcaud; Jean Quancard; Franck Teffo; Hervé Meudal; Marie-Claude Fournié-Zaluski; Bernard P Roques
Journal: J Med Chem Date: 2003-10-23 Impact factor: 7.446

6. Small tripeptide surrogates with low nanomolar affinity as potent inhibitors of the botulinum neurotoxin B metallo-proteolytic activity.

Authors: Armand Blommaert; Serge Turcaud; Christine Anne; Bernard P Roques
Journal: Bioorg Med Chem Date: 2004-06-01 Impact factor: 3.641

7. Fluorigenic substrates for the protease activities of botulinum neurotoxins, serotypes A, B, and F.

Authors: James J Schmidt; Robert G Stafford
Journal: Appl Environ Microbiol Date: 2003-01 Impact factor: 4.792

8. Clinical and laboratory comparison of botulism from toxin types A, B, and E in the United States, 1975-1988.

Authors: B A Woodruff; P M Griffin; L M McCroskey; J F Smart; R B Wainwright; R G Bryant; L C Hutwagner; C L Hatheway
Journal: J Infect Dis Date: 1992-12 Impact factor: 5.226

9. Thio-derived disulfides as potent inhibitors of botulinum neurotoxin type B: implications for zinc interaction.

Authors: Christine Anne; Armand Blommaert; Serge Turcaud; Anne-Sophie Martin; Hervé Meudal; Bernard P Roques
Journal: Bioorg Med Chem Date: 2003-10-15 Impact factor: 3.641

10. Botulinum neurotoxins are zinc proteins.

Authors: G Schiavo; O Rossetto; A Santucci; B R DasGupta; C Montecucco
Journal: J Biol Chem Date: 1992-11-25 Impact factor: 5.157

4 in total

1. A cross-over inhibitor of the botulinum neurotoxin light chain B: a natural product implicating an exosite mechanism of action.

Authors: Nicholas T Salzameda; Lisa M Eubanks; Joseph S Zakhari; Kyoji Tsuchikama; Nicholas J DeNunzio; Karen N Allen; Mark S Hixon; Kim D Janda
Journal: Chem Commun (Camb) Date: 2011-01-04 Impact factor: 6.222