Immobilization of glucose oxidase on a poly(dimethylsiloxane) layer by using poly(L-lysine) as a polymer backbone.

Poly(dimethylsiloxane) (PDMS)-coated platinum electrodes modified with glucose oxidase (GOx) have been prepared from poly(L-lysine) (polymer backbone), glutaraldehyde (cross-linking agent) and poly(ethylene glycol) units. To fabricate a GOx layer by applying cross-linking chemistries, the PDMS layer was treated with oxygen plasma to replace silane groups with silanol groups. Optimization for the chemical fabrication of a GOx layer resulted in a simple preparation of sensors with a wide detectable range (0.1-6.0 mM) and without interference from hydrogen peroxide produced by a GOx reaction and the other in biological samples.