Flow injection chemiluminescence determination of 6-mercaptopurine based on a new system of potassium permanganate-thioacetamide-sodium hexametaphosphate.

A novel chemiluminescence method for the determination of 6-mercaptopurine was established based on 6-mercaptopurine inhibition of the chemiluminescence emission of potassium permanganate-thioacetamide-sodium hexametaphosphate system. The peak height was proportional to log 6-mercaptopurine concentration in the range 7.0 × 10(-10) to 1.0 × 10(-7)  g/mL and the detection limit was 1.9 × 10(-11)  g/mL (S/N = 3). The relative standard deviation was 1.5% for the determination of 8.0 × 10(-8)  g/mL 6-mercaptopurine (n = 11). The proposed sensor was successfully applied to the analysis of 6-mercaptopurine in human serum samples.