Literature DB >> 1974244

Identification of beta 2-adrenoceptors on guinea pig alveolar macrophages using (-)-3-[125I]iodocyanopindolol.

R Leurs1, F D Beusenberg, A Bast, J G Van Amsterdam, H Timmerman.   

Abstract

The beta-adrenoceptor antagonist (-)-3-[125I]iodocyanopindolol ([125I]ICYP) binds with high affinity and in saturable way to membranes of guinea pig alveolar macrophages. The equilibrium dissociation constant for [125I]ICYP is 24.3 +/- 1.2 pM, and the number of binding sites is 166.3 +/- 13.7 fmol/mg protein (N = 4, +/- SEM). Displacement studies with selective antagonists showed that [125I]ICYP labels beta 2-adrenoceptors on guinea pig alveolar macrophages.

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Year:  1990        PMID： 1974244     DOI： 10.1007/bf00914093

Source DB:  PubMed          Journal:  Inflammation        ISSN： 0360-3997            Impact factor:   4.092


  10 in total

1.  Specific and non-specific effects of beta-adrenoceptor agonists on guinea pig alveolar macrophage function.

Authors:  P A Henricks; B Van Esch; A J Van Oosterhout; F P Nijkamp
Journal:  Eur J Pharmacol       Date:  1988-08-02       Impact factor: 4.432

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Regulation of cyclic AMP levels in alveolar macrophages of guinea pigs and man by prostanoids and beta-adrenergic agents.

Authors:  F D Beusenberg; M J Adolfs; J M van Schaik-van Groningen; H C Hoogsteden; I L Bonta
Journal:  Agents Actions       Date:  1989-01

4.  Pulmonary macrophages induce deterioration of guinea-pig tracheal beta-adrenergic function through release of oxygen radicals.

Authors:  F Engels; R S Oosting; F P Nijkamp
Journal:  Eur J Pharmacol       Date:  1985-04-23       Impact factor: 4.432

5.  Beta-adrenergic control of motility in the rat colon. II. Proportions of beta 1- and beta 2-adrenoceptors identified with 125I-(-)pindolol binding.

Authors:  B A Ek; S R Nahorski
Journal:  Gastroenterology       Date:  1986-02       Impact factor: 22.682

6.  Ligand: a versatile computerized approach for characterization of ligand-binding systems.

Authors:  P J Munson; D Rodbard
Journal:  Anal Biochem       Date:  1980-09-01       Impact factor: 3.365

Review 7.  Immunomodulatory-antiinflammatory functions of E-type prostaglandins. Minireview with emphasis on macrophage-mediated effects.

Authors:  I L Bonta; M J Parnham
Journal:  Int J Immunopharmacol       Date:  1982

8.  Identification and characterization of a homogeneous population of beta 2-adrenergic receptors on human alveolar macrophages.

Authors:  S B Liggett
Journal:  Am Rev Respir Dis       Date:  1989-02

9.  Effect of macrophage stimulation on parasympathetic airway contraction in dogs.

Authors:  J Tamaoki; K Sekizawa; I F Ueki; P D Graf; J A Nadel; T D Bigby
Journal:  Eur J Pharmacol       Date:  1987-06-26       Impact factor: 4.432

10.  (+/-)[125Iodo] cyanopindolol, a new ligand for beta-adrenoceptors: identification and quantitation of subclasses of beta-adrenoceptors in guinea pig.

Authors:  G Engel; D Hoyer; R Berthold; H Wagner
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1981       Impact factor: 3.000
  10 in total

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