| Literature DB >> 1973900 |
C J Thorns1, J A Sawtell, J A Miller, G W Wood.
Abstract
A monoclonal antibody (Mab)-based antigen capture enzyme-linked immunosorbent assay (ELISA) was developed and standardised for the detection of the K99 adhesin of Escherichia coli in aqueous vaccines. A repeating epitope on the K99 antigen is first captured then detected using peroxidase-labelled antibody. The assay gave positive results with all the vaccines tested that were known to contain K99 and was specific, rapid and reproducible.Entities:
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Year: 1990 PMID： 1973900 DOI： 10.1016/1045-1056(90)90020-z
Source DB: PubMed Journal: Biologicals ISSN： 1045-1056 Impact factor: 1.856