Pu-hua Bao1, Min Wang, Jun Su, Meng-yu Liu, Huan-xiang Zhang. 1. Department of Cell Biology, Medical College of Soochow University; Jiangsu Province Key Laboratory of Stem Cell Research, Suzhou 215123, China. baopuhua@163.com
Abstract
AIM: To investigate the tropism of bone marrow mesenchymal stem cells (BMSCs) in neurogenic differentiation for glioma and SDF-1alpha. METHODS: BHA, bFGF, and DMSO were used to induce the neural differentiation of BMSCs, and the expression of neural markers, Nestin, beta-III-Tubulin and NSE were analyzed by immunocytochemistry. We investigated, by using the Dunn chamber, the migration of BMSCs in neurogenic differentiation towards glioma and SDF-1alpha. RESULTS: BMSCs could be induced to differentiate into neuron-like cells, while the control remained the morphology of BMSCs. Dunn chamber analysis revealed that both the migration speed and efficiency of cells induced by C6 conditioned medium and SDF-1alpha were higher than control, demonstrating the chemotactic effect of both C6 conditioned medium and SDF-1alpha on BMSCs. These results were confimed by examination of migration tracks of individual cells. Moreover, BMSCs at different states of differentiation showed different degree of tropism for C6 conditioned medium and SDF-1alpha. CONCLUSION: The directed migration of BMSCs is closely related to the differentiation states of these cells.
AIM: To investigate the tropism of bone marrow mesenchymal stem cells (BMSCs) in neurogenic differentiation for glioma and SDF-1alpha. METHODS:BHA, bFGF, and DMSO were used to induce the neural differentiation of BMSCs, and the expression of neural markers, Nestin, beta-III-Tubulin and NSE were analyzed by immunocytochemistry. We investigated, by using the Dunn chamber, the migration of BMSCs in neurogenic differentiation towards glioma and SDF-1alpha. RESULTS: BMSCs could be induced to differentiate into neuron-like cells, while the control remained the morphology of BMSCs. Dunn chamber analysis revealed that both the migration speed and efficiency of cells induced by C6 conditioned medium and SDF-1alpha were higher than control, demonstrating the chemotactic effect of both C6 conditioned medium and SDF-1alpha on BMSCs. These results were confimed by examination of migration tracks of individual cells. Moreover, BMSCs at different states of differentiation showed different degree of tropism for C6 conditioned medium and SDF-1alpha. CONCLUSION: The directed migration of BMSCs is closely related to the differentiation states of these cells.