Ning Pan1, Ai-ran Zhang, Mao-sen Mu, Ying-chun Hou. 1. Lab of Tumor Cellular and Molecular Biology, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China. pnpanpan07@126.com
Abstract
AIM: To investigate the effect of siRNA targeting FAK gene on proliferation and motility of colorectal cancer. METHODS: Recombinant plasmids that produced siRNAs targeting FAK were designed and cloned, then transfected into Caco-2 cells. The changes of FAK expression levels were examined by RT-PCR and immunocytochemistry. The effects of FAK gene knockdown on apoptotic morphological changes, proliferation, and motility were investigated. RESULTS: Recombinant plasmids targeting FAK were successfully constructed, FAK mRNA and protein level was silenced in Caco-2 cells significantly. The inhibition of mRNA level was achieved maximal at 48 h post transfection with time dependent. The ability of proliferation and motility of Caco-2 cells were significantly decreased. CONCLUSION: Plasmid-mediated FAK siRNA could inhibit FAK gene expression. The proliferation, motility and apoptosis were inhibited effectively, which suggested that FAK expression is closely associated with the proliferation, motility and apoptosis, etc. The results may be used as the reference for gene therapy of colorectal cancer.
AIM: To investigate the effect of siRNA targeting FAK gene on proliferation and motility of colorectal cancer. METHODS: Recombinant plasmids that produced siRNAs targeting FAK were designed and cloned, then transfected into Caco-2 cells. The changes of FAK expression levels were examined by RT-PCR and immunocytochemistry. The effects of FAK gene knockdown on apoptotic morphological changes, proliferation, and motility were investigated. RESULTS: Recombinant plasmids targeting FAK were successfully constructed, FAK mRNA and protein level was silenced in Caco-2 cells significantly. The inhibition of mRNA level was achieved maximal at 48 h post transfection with time dependent. The ability of proliferation and motility of Caco-2 cells were significantly decreased. CONCLUSION: Plasmid-mediated FAK siRNA could inhibit FAK gene expression. The proliferation, motility and apoptosis were inhibited effectively, which suggested that FAK expression is closely associated with the proliferation, motility and apoptosis, etc. The results may be used as the reference for gene therapy of colorectal cancer.