Literature DB >> 1973271

A sensitive method for the assay of glutamine synthetase.

N Seiler, J Reid, B Knödgen.   

Abstract

The method for the assay of glutamine synthetase (GlnS) relies on the gamma-glutamyl transferase reaction, i.e. the formation of glutamyl-gamma-hydroxamate from glutamine and hydroxylamine, and the chromatographic separation of the reaction product from the reactants. The method is not only simple and reliable, but also has a sensitivity comparable to those methods applying radioactively labelled substrates. This new procedure has been applied to the assay of GlnS in cultured rat cortical astroglial cells which have been treated with a homologous series of alpha, omega-bis-(dimethylamino)alkanes. Effects of these drugs on astroglial development are reported.

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Year:  1990        PMID： 1973271     DOI： 10.1007/bf00968676

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  27 in total

1.  Regional distribution of putrescine, spermidine and spermine in relation to the distribution of RNA and DNA in the rat nervous system.

Authors:  N Seiler; T Schmidt-Glenewinkel
Journal:  J Neurochem       Date:  1975-04       Impact factor: 5.372

2.  N-permethylation of polyamines for gas chromatographic and mass spectrometric analyses.

Authors:  A G Giumanini; G Chiavari; F L Scarponi
Journal:  Anal Chem       Date:  1976-03       Impact factor: 6.986

3.  Determination of protein: a modification of the Lowry method that gives a linear photometric response.

Authors:  E F Hartree
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

4.  Cellular localization and regulation of glutamine synthetase in primary cultures of brain cells from newborn mice.

Authors:  K Hallermayer; C Harmening; B Hamprecht
Journal:  J Neurochem       Date:  1981-07       Impact factor: 5.372

5.  Glutamine synthetase from mammalian tissues.

Authors:  A Meister
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

6.  Binding of adenosine triphosphate and adenosine diphosphate by glutamine synthetase.

Authors:  V P Wellner; A Meister
Journal:  Biochemistry       Date:  1966-03       Impact factor: 3.162

7.  Regulation of glutamine synthetase synthesis and activity by glucocorticoids and adrenoceptor activation in astroglial cells.

Authors:  E Hansson
Journal:  Neurochem Res       Date:  1989-06       Impact factor: 3.996

8.  Astroglial cells: glucocorticoid target cells in the brain.

Authors:  M Tardy; B Rolland; C Fages; M Caldani
Journal:  Clin Neuropharmacol       Date:  1984       Impact factor: 1.592

9.  Glutamine synthetase and energy metabolism enzymes in cultured chick glial cells: modulation by dibutyryl cyclic AMP, hydrocortisone, and trypsinization.

Authors:  G Tholey; M Ledig; S Bloch; P Mandel
Journal:  Neurochem Res       Date:  1985-02       Impact factor: 3.996

10.  Cellular location of glutamine synthetase and lactate dehydrogenase in oligodendrocyte-enriched cultures from rat brain.

Authors:  R A Warringa; M F van Berlo; W Klein; M Lopes-Cardozo
Journal:  J Neurochem       Date:  1988-05       Impact factor: 5.372
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  2 in total

1.  Cytosolic Glutamine Synthetase Gln1;2 Is the Main Isozyme Contributing to GS1 Activity and Can Be Up-Regulated to Relieve Ammonium Toxicity.

Authors:  Miao Guan; Thomas C de Bang; Carsten Pedersen; Jan K Schjoerring
Journal:  Plant Physiol       Date:  2016-05-26       Impact factor: 8.340

2.  Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2.

Authors:  Yajie Gao; Thomas C de Bang; Jan K Schjoerring
Journal:  Plant Biotechnol J       Date:  2018-12-27       Impact factor: 9.803
  2 in total

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