Literature DB >> 19731225

Manipulation of mouse embryonic stem cells for knockout mouse production.

Advait Limaye1, Bradford Hall, Ashok B Kulkarni.   

Abstract

The establishment of mouse embryonic stem (ES) cell lines has allowed for the gene?ration of the knockout mouse. ES cells that are genetically altered in culture can then be manipulated to derive a whole mouse containing the desired mutation. To successfully generate a knockout mouse, however, the ES cells must be carefully cultivated in a pluripotent state throughout the gene-targeting experiment. This unit describes detailed step-by-step protocols, reagents, equipment, and strategies needed for the successful generation of gene knockout embryonic stem cells using homologous recombination technologies.

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Year:  2009        PMID： 19731225      PMCID： PMC2759096          DOI： 10.1002/0471143030.cb1913s44

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  46 in total

Review 1.  Technical assessment of the first 20 years of research using mouse embryonic stem cell lines.

Authors:  Gregory J Downing; James F Battey
Journal:  Stem Cells       Date:  2004       Impact factor: 6.277

2.  Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos.

Authors:  Guy S Eakin; Anna-Katerina Hadjantonakis
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

3.  Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution.

Authors:  Nimet Maherali; Rupa Sridharan; Wei Xie; Jochen Utikal; Sarah Eminli; Katrin Arnold; Matthias Stadtfeld; Robin Yachechko; Jason Tchieu; Rudolf Jaenisch; Kathrin Plath; Konrad Hochedlinger
Journal:  Cell Stem Cell       Date:  2007-06-07       Impact factor: 24.633

4.  Transgenesis by means of blastocyst-derived embryonic stem cell lines.

Authors:  A Gossler; T Doetschman; R Korn; E Serfling; R Kemler
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

5.  The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos.

Authors:  L C Stevens
Journal:  Dev Biol       Date:  1970-03       Impact factor: 3.582

6.  Efficient generation of transgenic BALB/c mice using BALB/c embryonic stem cells.

Authors:  A Dinkel; W K Aicher; K Warnatz; K Bürki; H Eibel; B Ledermann
Journal:  J Immunol Methods       Date:  1999-03-04       Impact factor: 2.303

7.  Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector.

Authors:  E Robertson; A Bradley; M Kuehn; M Evans
Journal:  Nature       Date:  1986 Oct 2-8       Impact factor: 49.962

8.  Generation of germline-competent induced pluripotent stem cells.

Authors:  Keisuke Okita; Tomoko Ichisaka; Shinya Yamanaka
Journal:  Nature       Date:  2007-06-06       Impact factor: 49.962

9.  In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.

Authors:  Marius Wernig; Alexander Meissner; Ruth Foreman; Tobias Brambrink; Manching Ku; Konrad Hochedlinger; Bradley E Bernstein; Rudolf Jaenisch
Journal:  Nature       Date:  2007-06-06       Impact factor: 49.962

10.  Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.

Authors:  A Nagy; J Rossant; R Nagy; W Abramow-Newerly; J C Roder
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-15       Impact factor: 11.205
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  9 in total

1.  Targeted Deletion of Hsf1, 2, and 4 Genes in Mice.

Authors:  Xiongjie Jin; Binnur Eroglu; Demetrius Moskophidis; Nahid F Mivechi
Journal:  Methods Mol Biol       Date:  2018

2.  Passenger Gene Mutations: Unwanted Guests in Genetically Modified Mice.

Authors:  Cheryl L Ackert-Bicknell; Clifford J Rosen
Journal:  J Bone Miner Res       Date:  2016-01-19       Impact factor: 6.741

3.  Targeted deletion of Hsf1, 2, and 4 genes in mice.

Authors:  Xiongjie Jin; Binnur Eroglu; Demetrius Moskophidis; Nahid F Mivechi
Journal:  Methods Mol Biol       Date:  2011

4.  CD6 as a potential target for treating multiple sclerosis.

Authors:  Yan Li; Nora G Singer; Joy Whitbred; Michael A Bowen; David A Fox; Feng Lin
Journal:  Proc Natl Acad Sci U S A       Date:  2017-02-16       Impact factor: 11.205

5.  Gene expression analysis to identify mechanisms underlying heart failure susceptibility in mice and humans.

Authors:  Christoph Koentges; Mark E Pepin; Carolyn Müsse; Katharina Pfeil; Sonia V Viteri Alvarez; Natalie Hoppe; Michael M Hoffmann; Katja E Odening; Samuel Sossalla; Andreas Zirlik; Lutz Hein; Christoph Bode; Adam R Wende; Heiko Bugger
Journal:  Basic Res Cardiol       Date:  2017-12-29       Impact factor: 17.165

Review 6.  Recent molecular approaches to understanding astrocyte function in vivo.

Authors:  David Davila; Karine Thibault; Todd A Fiacco; Cendra Agulhon
Journal:  Front Cell Neurosci       Date:  2013-12-24       Impact factor: 5.505

7.  N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors level in hfat-1 transgenic pigs.

Authors:  Xingxing Liu; Daxin Pang; Ting Yuan; Zhuang Li; Zhanjun Li; Mingjun Zhang; Wenzhi Ren; Hongsheng Ouyang; Xiaochun Tang
Journal:  Lipids Health Dis       Date:  2016-05-10       Impact factor: 3.876

Review 8.  Mouse Models of Human Claudin-Associated Disorders: Benefits and Limitations.

Authors:  Murat Seker; Carmen Fernandez-Rodriguez; Luis Alfonso Martinez-Cruz; Dominik Müller
Journal:  Int J Mol Sci       Date:  2019-11-05       Impact factor: 5.923

Review 9.  Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9.

Authors:  Ferheen Abbasi; Haruhiko Miyata; Masahito Ikawa
Journal:  Reprod Med Biol       Date:  2017-10-14
  9 in total

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