Literature DB >> 19731047

Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies.

Pei Zhou1, Gerhard Wagner.   

Abstract

Although the rapid progress of NMR technology has significantly expanded the range of NMR-trackable systems, preparation of NMR-suitable samples that are highly soluble and stable remains a bottleneck for studies of many biological systems. The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological systems previously deemed unapproachable by solution NMR techniques. In this review, we provide a brief survey of the development and successful applications of the SET strategy in biomolecular NMR.We also comment on the criteria for choosing optimal SETs, such as for differently charged target proteins, and recent new developments on NMR-invisible SETs.

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Year:  2010        PMID： 19731047      PMCID： PMC2879018          DOI： 10.1007/s10858-009-9371-6

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  57 in total

1.  Predicting the solubility of recombinant proteins in Escherichia coli.

Authors:  D L Wilkinson; R G Harrison
Journal:  Biotechnology (N Y)       Date:  1991-05

2.  A simple method for improving protein solubility and long-term stability.

Authors:  Alexander P Golovanov; Guillaume M Hautbergue; Stuart A Wilson; Lu-Yun Lian
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3.  An efficient system for small protein expression and refolding.

Authors:  Yuan Cheng; Dinshaw J Patel
Journal:  Biochem Biophys Res Commun       Date:  2004-04-30       Impact factor: 3.575

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Authors:  Takuhiro Ito; Gerhard Wagner
Journal:  J Biomol NMR       Date:  2004-04       Impact factor: 2.835

5.  Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.

Authors:  C di Guan; P Li; P D Riggs; H Inouye
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

6.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Authors:  D B Smith; K S Johnson
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

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Authors:  Susan S Safadi; Gary S Shaw
Journal:  Biochemistry       Date:  2007-11-16       Impact factor: 3.162

8.  Solution structure of human initiation factor eIF2alpha reveals homology to the elongation factor eEF1B.

Authors:  Takuhiro Ito; Assen Marintchev; Gerhard Wagner
Journal:  Structure       Date:  2004-09       Impact factor: 5.006

9.  Enhanced in vitro refolding of insulin-like growth factor I using a solubilizing fusion partner.

Authors:  E Samuelsson; T Moks; B Nilsson; M Uhlen
Journal:  Biochemistry       Date:  1994-04-12       Impact factor: 3.162

10.  NMR structure of the apoptosis- and inflammation-related NALP1 pyrin domain.

Authors:  Sebastian Hiller; Andreas Kohl; Francesco Fiorito; Torsten Herrmann; Gerhard Wider; Jürg Tschopp; Markus G Grütter; Kurt Wüthrich
Journal:  Structure       Date:  2003-10       Impact factor: 5.006
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  39 in total

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Authors:  Matthew D Daugherty; David S Booth; Bhargavi Jayaraman; Yifan Cheng; Alan D Frankel
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Journal:  J Biol Chem       Date:  2020-04-28       Impact factor: 5.157

4.  Structural basis of ubiquitin recognition by translesion synthesis DNA polymerase ι.

Authors:  Gaofeng Cui; Robert C Benirschke; Han-Fang Tuan; Nenad Juranić; Slobodan Macura; Maria Victoria Botuyan; Georges Mer
Journal:  Biochemistry       Date:  2010-11-04       Impact factor: 3.162

5.  A topical issue: production and labeling of biological macromolecules for NMR investigations.

Authors:  Gerhard Wagner
Journal:  J Biomol NMR       Date:  2010-01       Impact factor: 2.835

6.  A novel and highly specific phage endolysin cell wall binding domain for detection of Bacillus cereus.

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Journal:  Eur Biophys J       Date:  2015-06-05       Impact factor: 1.733

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Journal:  Biochemistry       Date:  2013-03-20       Impact factor: 3.162

8.  Identification of the first small-molecule inhibitor of the REV7 DNA repair protein interaction.

Authors:  Marcelo L Actis; Nigus D Ambaye; Benjamin J Evison; Youming Shao; Murugendra Vanarotti; Akira Inoue; Ezelle T McDonald; Sotaro Kikuchi; Richard Heath; Kodai Hara; Hiroshi Hashimoto; Naoaki Fujii
Journal:  Bioorg Med Chem       Date:  2016-07-16       Impact factor: 3.641

9.  Intracellular pH modulates quinary structure.

Authors:  Rachel D Cohen; Alex J Guseman; Gary J Pielak
Journal:  Protein Sci       Date:  2015-08-30       Impact factor: 6.725

10.  The LcrG Tip Chaperone Protein of the Yersinia pestis Type III Secretion System Is Partially Folded.

Authors:  Sukanya Chaudhury; Clarice de Azevedo Souza; Gregory V Plano; Roberto N De Guzman
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