Literature DB >> 19729262

A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae.

Katja A Koskela1, Pirjo Matero, Janet M Blatny, Else M Fykse, Jaran Strand Olsen, Lasse O Nuotio, Simo Nikkari.   

Abstract

We report a multiplatform real-time polymerase chain reaction methodology based on genes encoding for the regulatory toxR activator and enterotoxin A protein to determine enterotoxigenic Vibrio cholerae types from other vibrios. This assay, which was successfully validated on a collection of 87 bacterial strains, including 63 representatives of V. cholerae and 8 noncholera vibrios provides a rapid tool for detection and identification of cholera.

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Year:  2009        PMID: 19729262     DOI: 10.1016/j.diagmicrobio.2009.07.009

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN: 0732-8893            Impact factor:   2.803


  3 in total

1.  A novel triplex quantitative PCR strategy for quantification of toxigenic and nontoxigenic Vibrio cholerae in aquatic environments.

Authors:  Rupert Bliem; Sonja Schauer; Helga Plicka; Adelheid Obwaller; Regina Sommer; Adolf Steinrigl; Munirul Alam; Georg H Reischer; Andreas H Farnleitner; Alexander Kirschner
Journal:  Appl Environ Microbiol       Date:  2015-02-27       Impact factor: 4.792

2.  Development and Validation of a Novel Real-time Assay for the Detection and Quantification of Vibrio cholerae.

Authors:  Ridwan Bin Rashid; Jannatul Ferdous; Suhella Tulsiani; Peter Kjaer Mackie Jensen; Anowara Begum
Journal:  Front Public Health       Date:  2017-05-19

3.  Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques.

Authors:  Anthony M Smith; Berthe-Marie Njanpop-Lafourcade; Martin A Mengel; Bradford D Gessner; Delphine Sauvageot; Bawimodom Bidjada; Berthe N Miwanda; Diallo M Saliou; Adèle Kacou N'Douba; José P Langa; Husna Ismail; Nomsa Tau; Arvinda Sooka; Karen H Keddy
Journal:  PLoS One       Date:  2015-11-25       Impact factor: 3.240

  3 in total

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