Kun Qi1, Fu-rong Deng, Xin-biao Guo. 1. Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
Abstract
OBJECTIVE: To investigate the effects of nanoscale titanium dioxide (Nano-TiO(2)) on gap junctional intercellular communication (GJIC) between human lung fibroblasts. METHODS: Human lung fibroblasts were exposed to two different sizes Nano-TiO(2) (25 nm and 80 nm) for 24 hours at the concentrations of 0, 10, 20, 40 and 80 mg/L, respectively. The GJIC between cells was measured by using fluorescence redistribution after photobleaching (FRAP) assay. RESULTS: The results showed that GJIC between cells was significantly inhibited both by 25 nm and 80 nm Nano-TiO(2) in a dose-dependent manner. The fluorescence recovery rate was 20.81%+/-1.93% in control group, 7.26%+/-0.91% (P<0.05) in 25 nm 80 mg/L group and 9.94%+/-2.08% (P<0.05) in 80 nm 80 mg/L group. At the same concentration, the inhibitory effect of 25 nm Nano-TiO(2) on GJIC was slightly stronger than that of 80 nm Nano-TiO(2). CONCLUSION: Nano-TiO(2) with different sizes can inhibit GJIC between human lung fibroblasts.
OBJECTIVE: To investigate the effects of nanoscale titanium dioxide (Nano-TiO(2)) on gap junctional intercellular communication (GJIC) between human lung fibroblasts. METHODS:Human lung fibroblasts were exposed to two different sizes Nano-TiO(2) (25 nm and 80 nm) for 24 hours at the concentrations of 0, 10, 20, 40 and 80 mg/L, respectively. The GJIC between cells was measured by using fluorescence redistribution after photobleaching (FRAP) assay. RESULTS: The results showed that GJIC between cells was significantly inhibited both by 25 nm and 80 nm Nano-TiO(2) in a dose-dependent manner. The fluorescence recovery rate was 20.81%+/-1.93% in control group, 7.26%+/-0.91% (P<0.05) in 25 nm 80 mg/L group and 9.94%+/-2.08% (P<0.05) in 80 nm 80 mg/L group. At the same concentration, the inhibitory effect of 25 nm Nano-TiO(2) on GJIC was slightly stronger than that of 80 nm Nano-TiO(2). CONCLUSION: Nano-TiO(2) with different sizes can inhibit GJIC between human lung fibroblasts.