OBJECTIVE: To study the effects of dexamethasone on intracellular expression of Th17 cytokine interleukin 17 and the mechanisms in asthmatic mice. METHODS: Experimental asthma was induced by ovalbumin (OVA) sensitization in 20 in female Balb/c mice with (dexamethasone group, n=10) or without dexamethasone treatment (model group, n=10), with another 10 serving as the control group. The levels of IL-17 in the bronchoalveolar lavage fluid (BALF) and serum of the mice were measured by enzyme-linked immunosorbent assay (ELISA), and the airway inflammation was evaluated by HE staining. The expressions of IL-17 and RORgammat mRNA were measured by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of RORgammat protein was measured by immunohistochemical staining. RESULTS: The levels of RORgammat and IL-17 mRNA and protein in the asthmatic model group were significantly higher than those in the control group (P<0.01), and the increased expressions of RORgammat and IL-17 mRNA and protein in the asthmatic mice were significantly reduced by dexamethasone treatment (P<0.05). CONCLUSION: Dexamethasone can inhibit the release of IL-17 probably by inhibiting RORgammat expression and blocking Th17 differentiation in asthmatic mice.
OBJECTIVE: To study the effects of dexamethasone on intracellular expression of Th17 cytokine interleukin 17 and the mechanisms in asthmatic mice. METHODS: Experimental asthma was induced by ovalbumin (OVA) sensitization in 20 in female Balb/c mice with (dexamethasone group, n=10) or without dexamethasone treatment (model group, n=10), with another 10 serving as the control group. The levels of IL-17 in the bronchoalveolar lavage fluid (BALF) and serum of the mice were measured by enzyme-linked immunosorbent assay (ELISA), and the airway inflammation was evaluated by HE staining. The expressions of IL-17 and RORgammat mRNA were measured by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of RORgammat protein was measured by immunohistochemical staining. RESULTS: The levels of RORgammat and IL-17 mRNA and protein in the asthmatic model group were significantly higher than those in the control group (P<0.01), and the increased expressions of RORgammat and IL-17 mRNA and protein in the asthmatic mice were significantly reduced by dexamethasone treatment (P<0.05). CONCLUSION:Dexamethasone can inhibit the release of IL-17 probably by inhibiting RORgammat expression and blocking Th17 differentiation in asthmatic mice.
Authors: Nir Yosef; Alex K Shalek; Jellert T Gaublomme; Hulin Jin; Youjin Lee; Amit Awasthi; Chuan Wu; Katarzyna Karwacz; Sheng Xiao; Marsela Jorgolli; David Gennert; Rahul Satija; Arvind Shakya; Diana Y Lu; John J Trombetta; Meenu R Pillai; Peter J Ratcliffe; Mathew L Coleman; Mark Bix; Dean Tantin; Hongkun Park; Vijay K Kuchroo; Aviv Regev Journal: Nature Date: 2013-03-06 Impact factor: 49.962
Authors: Rebecca A Martin; Jennifer L Ather; Rebecca Daggett; Laura Hoyt; John F Alcorn; Benjamin T Suratt; Daniel J Weiss; Lennart K A Lundblad; Matthew E Poynter Journal: PLoS One Date: 2013-09-19 Impact factor: 3.240