Gel-based proteomics approach to the study of metabolic changes in pear tissue during storage.

The effect of extreme gas conditions (anoxia and air) on the protein expression profiles of Conference pear slices was assessed with a differential gel electrophoresis (DIGE) approach using robust statistical analysis. Changes in expression, up to 4-fold, were identified in proteins involved in respiration, protein synthesis, and defense mechanisms. In addition, short-term exposure of pear slices to anoxia clearly induced up-regulation of transketolase and polygalacturonase inhibiting protein and down-regulation of several isoforms of the major allergen Pyrc (PR proteins), providing further evidence of the possible involvement of these enzymes in the development of the physiological disorder core breakdown. The role of these PR proteins under anoxia is unknown, but our results suggest that these proteins are involved in protection against abiotic stress such as the anoxic conditions applied.