Dyadobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from soil.

A Gram-negative, aerobic bacterium, designated strain BZ26(T), was isolated from hydrocarbon-contaminated soil. The strain was psychrophilic, showing good growth over a temperature range of 1-20 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ26(T) was related to members of the genus Dyadobacter and had highest 16S rRNA gene sequence similarity to Dyadobacter alkalitolerans 12116(T) (98.1 %), Dyadobacter koreensis KCTC 12537(T) (97.5 %) and Dyadobacter ginsengisoli Gsoil 043(T) (97.2 %). Strain BZ26(T) had MK-7 as the major menaquinone and summed feature 3 (C(16 : 1)omega7c/iso-C(15 : 0) 2-OH), C(16 : 1)omega5c and iso-C(15 : 0) as major fatty acids. The genomic DNA G+C content of strain BZ26(T) was 48.9 mol%. On the basis of phenotypic characteristics and genotypic analysis, strain BZ26(T) is considered to represent a novel species of the genus Dyadobacter, for which the name Dyadobacter psychrophilus sp. nov. is proposed. The type strain is BZ26(T) (=DSM 22270(T) =CGMCC 1.8951(T)).