OBJECTIVE: This study evaluated transenamel and transdentinal cytotoxic effects of a bleaching gel on the MDPC-23 cell line. STUDY DESIGN: Discs obtained from bovine incisors were placed in a metallic device to simulate an in vivo pulp chamber. Groups were formed according to the enamel surface treatment: G1: 35% H(2)O(2) bleaching gel; G2: 35% H(2)O(2) bleaching gel + halogen light; G3: halogen light; and G4: control. Cell metabolism was evaluated by the methyltetrazolium assay and cell morphology by scanning electron microscopy. RESULTS: Cell metabolism decreased by 31.7%, 41.6%, and 11.5% in G1, G2, and G3, respectively. Cytotoxic effects observed in G2 were significantly more severe compared with G3 and G4. In G1 and G2, a smaller number of viable cells with major morphologic alterations remained adhered to dentin. CONCLUSION: The bleaching gel associated with light presented transenamel and transdentinal cytotoxic effects characterised by direct damage to odontoblasts and decrease of their metabolic activity.
OBJECTIVE: This study evaluated transenamel and transdentinal cytotoxic effects of a bleaching gel on the MDPC-23 cell line. STUDY DESIGN: Discs obtained from bovine incisors were placed in a metallic device to simulate an in vivo pulp chamber. Groups were formed according to the enamel surface treatment: G1: 35% H(2)O(2) bleaching gel; G2: 35% H(2)O(2) bleaching gel + halogen light; G3: halogen light; and G4: control. Cell metabolism was evaluated by the methyltetrazolium assay and cell morphology by scanning electron microscopy. RESULTS: Cell metabolism decreased by 31.7%, 41.6%, and 11.5% in G1, G2, and G3, respectively. Cytotoxic effects observed in G2 were significantly more severe compared with G3 and G4. In G1 and G2, a smaller number of viable cells with major morphologic alterations remained adhered to dentin. CONCLUSION: The bleaching gel associated with light presented transenamel and transdentinal cytotoxic effects characterised by direct damage to odontoblasts and decrease of their metabolic activity.
Authors: Adriano Fonseca Lima; Ana Paula Dias Ribeiro; Fernanda Gonçalves Basso; Vanderlei Salvador Bagnato; Josimeri Hebling; Giselle Maria Marchi; Carlos Alberto de Souza Costa Journal: Lasers Med Sci Date: 2013-03-23 Impact factor: 3.161
Authors: Diana Gabriela Soares; Ana Paula Dias Ribeiro; Fernanda da Silveira Vargas; Josimeri Hebling; Carlos Alberto de Souza Costa Journal: Clin Oral Investig Date: 2012-12-06 Impact factor: 3.573
Authors: Taciana Marco Ferraz Caneppele; Carlos Rocha Gomes Torres; Maria Filomena Rocha Lima Huhtala; Eduardo Bresciani Journal: ScientificWorldJournal Date: 2015-03-17
Authors: Carlos R G Torres; Cristiane S Souza; Alessandra B Borges; Maria Filomena R L Huhtala; Taciana M F Caneppele Journal: ScientificWorldJournal Date: 2013-09-18