BACKGROUND: Dengue (DEN) viruses have become a public health problem that affects approximately 100 million people worldwide each year. Prevention measures rely on vector control programs, which are inefficient. Therefore, a vaccine is urgently needed. METHODS: The main goal of our laboratory is to develop an efficient tetravalent DEN DNA vaccine. In this study, we constructed four DEN-2 DNA vaccines expressing prM/env genes, using the homologous leader sequence (VecD2, VRD2E) or the tissue plasminogen activator (tPA) secretory signal (VecD2tpa, VRD2tpa). In vitro expression was tested by transient transfections and Western blot. The immunogenicity and protective efficacy of the vaccine candidates was evaluated in BALB/c mice, using intramuscular (IM) and intradermal (ID) vaccination routes. RESULTS: Envelope (E) protein expression was detected in transfected COS-7 or 293T cells. We found statistical differences in the antibody responses induced by these vaccine candidates. In addition, the strongest antibody responses and protection were observed when the vaccines were delivered intramuscularly. Moreover, the tPA leader sequence did not significantly improve the vaccine immunogenicity since VecD2 and VecD2tpa induced similar antibody responses. CONCLUSIONS: We demonstrated that most of our DNA vaccine candidates could induce antibody responses and partial protection against DEN-2 virus in mice. These results provide valuable information for the design and construction of a tetravalent DEN DNA vaccine.
BACKGROUND: Dengue (DEN) viruses have become a public health problem that affects approximately 100 million people worldwide each year. Prevention measures rely on vector control programs, which are inefficient. Therefore, a vaccine is urgently needed. METHODS: The main goal of our laboratory is to develop an efficient tetravalent DEN DNA vaccine. In this study, we constructed four DEN-2 DNA vaccines expressing prM/env genes, using the homologous leader sequence (VecD2, VRD2E) or the tissue plasminogen activator (tPA) secretory signal (VecD2tpa, VRD2tpa). In vitro expression was tested by transient transfections and Western blot. The immunogenicity and protective efficacy of the vaccine candidates was evaluated in BALB/c mice, using intramuscular (IM) and intradermal (ID) vaccination routes. RESULTS: Envelope (E) protein expression was detected in transfected COS-7 or 293T cells. We found statistical differences in the antibody responses induced by these vaccine candidates. In addition, the strongest antibody responses and protection were observed when the vaccines were delivered intramuscularly. Moreover, the tPA leader sequence did not significantly improve the vaccine immunogenicity since VecD2 and VecD2tpa induced similar antibody responses. CONCLUSIONS: We demonstrated that most of our DNA vaccine candidates could induce antibody responses and partial protection against DEN-2 virus in mice. These results provide valuable information for the design and construction of a tetravalent DEN DNA vaccine.
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Authors: Wellington Sun; Dennis Cunningham; Steven S Wasserman; Judith Perry; J Robert Putnak; Kenneth H Eckels; David W Vaughn; Stephen J Thomas; Niranjan Kanesa-Thasan; Bruce L Innis; Robert Edelman Journal: Hum Vaccin Date: 2009-01-27
Authors: J Robert Putnak; Beth-Ann Coller; Gerald Voss; David W Vaughn; David Clements; Iain Peters; Gary Bignami; Hou-Shu Houng; Robert C-M Chen; David A Barvir; Jitvimol Seriwatana; Sylvie Cayphas; Nathalie Garçon; Dirk Gheysen; Niranjan Kanesa-Thasan; Mike McDonell; Tom Humphreys; Kenneth H Eckels; Jean-Paul Prieels; Bruce L Innis Journal: Vaccine Date: 2005-08-15 Impact factor: 3.641
Authors: Julie H McArthur; Anna P Durbin; Jennifer A Marron; Kimberli A Wanionek; Bhavin Thumar; Dennis J Pierro; Alexander C Schmidt; Joseph E Blaney; Brian R Murphy; Stephen S Whitehead Journal: Am J Trop Med Hyg Date: 2008-11 Impact factor: 2.345
Authors: Monica Poggianella; José L Slon Campos; Kuan Rong Chan; Hwee Cheng Tan; Marco Bestagno; Eng Eong Ooi; Oscar R Burrone Journal: PLoS Negl Trop Dis Date: 2015-07-28