Construction and expression of a recombinant fibronectinIII10 protein for integrin-mediated cell adhesion.

Specific sequences of cell-adhesive peptide/proteins are often required for the bio-inert synthetic polymers to improve cell adhesion. We have developed a recombinant fibronectin fragment (FN(III)10), the central cell-binding domain containing RGD motif, to endow biomaterial surfaces with abilities to promote an integrin-mediated cell adhesion. Immobilized FN(III)10 stimulated adhesion of MC3TC-E1 cells in an integrin-dependent manner.