| Literature DB >> 1970548 |
L van de Zande1, W T Labruyère, A C Arnberg, R H Wilson, A J van den Bogaert, A T Das, D A van Oorschot, C Frijters, R Charles, A F Moorman.
Abstract
From a rat genomic library in phage lambda Charon4A, a complete glutamine synthetase-encoding gene was isolated. The gene is 9.5-10 kb long, consists of seven exons, and codes for two mRNA species of 1375 nucleotides (nt) and 2787 nt, respectively. For both mRNAs, full-length cDNAs containing a short poly(A) tract were identified. The sequences of the entire mRNA and of the exon-intron transitions were determined. The smaller mRNA is identical to the 5' 1375 nt of the long mRNA and contains the entire protein-coding region. The position of the transcription start point was mapped. Within the first 118 bp of promoter sequence, a (T)ATAA-box, a CCAAT-box and an SP1-binding site were identified.Entities:
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Year: 1990 PMID： 1970548 DOI： 10.1016/0378-1119(90)90306-c
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688