Simultaneous real-time assay of copper and cadmium ions by infrared photo diode electrode implanted in the muscle of live fish.

The electrical circuit of an infrared photodiode electrode (IPE) was used in the simultaneous assay of copper and cadmium ions. The electrode's cyclic voltammetry (CV), chronoamperometry and square-wave (SW) stripping voltammetric optimum conditions were examined. Results for 0-160 mg L(-1) and 50-400 microg L(-1) SW Cu(II) Cd(II), the relative standard deviation of 0.158 Cu(II), 0.077 Cd(II) (n = 15) using 20.0 mg L(-1) have been obtained at optimum conditions. The low detection limit (S/N) was attained to be at 14.71 microg L(-1)(2.31 x 10(-7) mol L(-1)) Cu(II) and 18.42 microg L(-1)(1.63 x 10(-7) mol L(-1)) Cd(II). The handmade electrode was implanted deep in the muscle of live fish and interfaced with an electrochemical workstation. Real-time analytical application was performed on the online assay of living tissue as the specimen was moving. The methods are deemed useful in interfaced assay for physiological control, nanodiode fabrication, and in the production of laboratory on a biochip. 2009 Wiley Periodicals, Inc.