OBJECTIVES: The aim was to test the potential use of an extract of Mikania laevigata (popularly known in Brazil as guaco), made from leaves harvested in different months of the year, on neutrophil migration after an inflammatory stimulus and investigate the underlying molecular mechanisms. METHODS: We examined the effect of guaco on vascular permeability and leucocyte function in carrageenan-induced peritonitis in mice. KEY FINDINGS: Our results demonstrated that guaco extract administered subcutaneously (3 mg/kg) decreased the vascular permeability and also leucocyte rolling and adhesion to the inflamed tissues by a mechanism dependent on nitric oxide. Specifically, inhibitors of nitric oxide synthase remarkably abrogated the guaco extract-mediated suppression of neutrophil migration to the inflammatory site. In addition, guaco extract-mediated suppression of neutrophil migration appeared to be dependent on the production of the cytokines interleukin-1beta and tumour necrosis factor-alpha. One of the major constituents of the guaco extract, coumarin, was able to inhibit the neutrophil migration towards the inflammatory focus. CONCLUSIONS: In conclusion the anti-inflammatory effect induced by guaco extract may be by inhibition of pro-inflammatory cytokine production at the inflammatory site.
OBJECTIVES: The aim was to test the potential use of an extract of Mikania laevigata (popularly known in Brazil as guaco), made from leaves harvested in different months of the year, on neutrophil migration after an inflammatory stimulus and investigate the underlying molecular mechanisms. METHODS: We examined the effect of guaco on vascular permeability and leucocyte function in carrageenan-induced peritonitis in mice. KEY FINDINGS: Our results demonstrated that guaco extract administered subcutaneously (3 mg/kg) decreased the vascular permeability and also leucocyte rolling and adhesion to the inflamed tissues by a mechanism dependent on nitric oxide. Specifically, inhibitors of nitric oxide synthase remarkably abrogated the guaco extract-mediated suppression of neutrophil migration to the inflammatory site. In addition, guaco extract-mediated suppression of neutrophil migration appeared to be dependent on the production of the cytokines interleukin-1beta and tumour necrosis factor-alpha. One of the major constituents of the guaco extract, coumarin, was able to inhibit the neutrophil migration towards the inflammatory focus. CONCLUSIONS: In conclusion the anti-inflammatory effect induced by guaco extract may be by inhibition of pro-inflammatory cytokine production at the inflammatory site.
Authors: Hina Fatima; Muhammad Shahid; Chris Pruitt; Meredith A Pung; Paul J Mills; Muhammad Riaz; Rizwan Ashraf Journal: Antioxidants (Basel) Date: 2022-02-11
Authors: Claudiney de Freitas Alves; Giovanna Natalia Angeli; Daniely Cornélio Favarin; Edinéia Lemos de Andrade; Javier Emilio Lazo Chica; Lúcia Helena Faccioli; Paulo Roberto da Silva; Alexandre de Paula Rogerio Journal: Mediators Inflamm Date: 2013-11-26 Impact factor: 4.711
Authors: Bruno B Benatti; Jozafá C Campos-Júnior; Vilmar J Silva-Filho; Polyanna M Alves; Isabela R Rodrigues; Elizabeth Uber-Bucek; Silvio M Vieira; Marcelo H Napimoga Journal: J Appl Oral Sci Date: 2012 May-Jun Impact factor: 2.698