BACKGROUND: Patients with tsutsugamushi disease sometimes die if they do not receive appropriate chemotherapy. This study measured the concentration of several cytokines both before and after the administration of tetracyclines, and evaluated the changes in cytokine levels in patient serum to investigate the relationship between serum levels of cytokines and disease severity. METHODS: A total of nine patients were infected with Orientia tsutsugamushi. The diagnosis of tsutsugamushi disease was made using an indirect immunoperoxidase antibody test. The serum concentrations of cytokines were measured using enzyme-linked immunosorbent assays. RESULTS: The levels of interleukin (IL)-10 (mean 71.7 pg/ml) and IL-12p40 (mean 588 pg/ml) were elevated in all patients in the acute phase, above the normal upper limits. Tumor necrosis factor-alpha (TNF-alpha) levels (mean 9.20 pg/ml) were elevated in 89% and interferon-gamma (IFN-gamma) levels (mean 41.0 pg/ml) in 44% of patients. The down-regulation of these overproduced cytokines was observed after chemotherapy. There was a significant correlation between the concentrations of TNF-alpha in the acute phase and the severity of disease (r=0.918). CONCLUSION: The concentration of TNF-alpha may predict the severity of tsutsugamushi disease in the acute infectious phase.
BACKGROUND:Patients with tsutsugamushi disease sometimes die if they do not receive appropriate chemotherapy. This study measured the concentration of several cytokines both before and after the administration of tetracyclines, and evaluated the changes in cytokine levels in patient serum to investigate the relationship between serum levels of cytokines and disease severity. METHODS: A total of nine patients were infected with Orientia tsutsugamushi. The diagnosis of tsutsugamushi disease was made using an indirect immunoperoxidase antibody test. The serum concentrations of cytokines were measured using enzyme-linked immunosorbent assays. RESULTS: The levels of interleukin (IL)-10 (mean 71.7 pg/ml) and IL-12p40 (mean 588 pg/ml) were elevated in all patients in the acute phase, above the normal upper limits. Tumor necrosis factor-alpha (TNF-alpha) levels (mean 9.20 pg/ml) were elevated in 89% and interferon-gamma (IFN-gamma) levels (mean 41.0 pg/ml) in 44% of patients. The down-regulation of these overproduced cytokines was observed after chemotherapy. There was a significant correlation between the concentrations of TNF-alpha in the acute phase and the severity of disease (r=0.918). CONCLUSION: The concentration of TNF-alpha may predict the severity of tsutsugamushi disease in the acute infectious phase.
Authors: Pasquale Mansueto; Giustina Vitale; Antonio Cascio; Aurelio Seidita; Ilenia Pepe; Antonio Carroccio; Salvatore di Rosa; Giovam Battista Rini; Enrico Cillari; David H Walker Journal: Clin Dev Immunol Date: 2011-09-06
Authors: Estela Costa; Richard Re Uwiera; John P Kastelic; L Brent Selinger; G Douglas Inglis Journal: Gut Pathog Date: 2011-09-25 Impact factor: 4.181
Authors: Lynn Soong; Hui Wang; Thomas R Shelite; Yuejin Liang; Nicole L Mendell; Jiaren Sun; Bin Gong; Gustavo A Valbuena; Donald H Bouyer; David H Walker Journal: PLoS Negl Trop Dis Date: 2014-09-25
Authors: Elisabeth Astrup; Jeshina Janardhanan; Kari Otterdal; Thor Ueland; John A J Prakash; Tove Lekva; Øystein A Strand; O C Abraham; Kurien Thomas; Jan Kristian Damås; Prasad Mathews; Dilip Mathai; Pål Aukrust; George M Varghese Journal: PLoS Negl Trop Dis Date: 2014-02-06